Kanazawa Masahiro, Ando Satoko, Tsuda Michio, Suzuki Toshiyasu
Department of Anesthesiology, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
J Nutr Sci Vitaminol (Tokyo). 2010;56(2):117-22. doi: 10.3177/jnsv.56.117.
An infusion of amino acids stimulates heat production in skeletal muscle and then attenuates the anesthesia-induced hypothermia. However, in a clinical setting, some patients have atrophic skeletal muscle caused by various factors. The present study was therefore conducted to investigate the effect of amino acids on the anesthesia-induced hypothermia in the state of muscle atrophy. As the muscle atrophy model, Sprague-Dawley rats were subjected to hindlimb immobilization for 2 wk. Normal rats and atrophy model rats were randomly assigned to one of the two treatment groups: saline or amino acids (n=8 for each group). Test solutions were administered intravenously to the rats under sevoflurane anesthesia for 180 min, and the rectal temperature was measured. Plasma samples were collected for measurement of insulin, blood glucose, and free amino acids. The rectal temperature was significantly higher in the normal-amino acid group than in the muscle atrophy-amino acid group from 75 to 180 min. The plasma insulin level was significantly higher in the rats given amino acids than in the rats given saline in both normal and model groups. In the rats given amino acids, plasma total free amino acid concentration was higher in the model group than in the normal group. These results indicate that skeletal muscle plays an important role in changes in body temperature during anesthesia and the effect of amino acids on anesthesia-induced hypothermia decreases in the muscle atrophy state. In addition, intravenous amino acids administration during anesthesia induces an increase in the plasma insulin level.
输注氨基酸可刺激骨骼肌产热，进而减轻麻醉诱导的体温过低。然而，在临床环境中，一些患者因各种因素导致骨骼肌萎缩。因此，本研究旨在探讨在肌肉萎缩状态下氨基酸对麻醉诱导体温过低的影响。作为肌肉萎缩模型，将Sprague-Dawley大鼠后肢固定2周。将正常大鼠和萎缩模型大鼠随机分为两个治疗组之一：生理盐水组或氨基酸组（每组n = 8）。在七氟醚麻醉下，将测试溶液静脉注射给大鼠180分钟，并测量直肠温度。采集血浆样本以测量胰岛素、血糖和游离氨基酸。在75至180分钟期间，正常-氨基酸组的直肠温度显著高于肌肉萎缩-氨基酸组。在正常组和模型组中，给予氨基酸的大鼠血浆胰岛素水平均显著高于给予生理盐水的大鼠。在给予氨基酸的大鼠中，模型组的血浆总游离氨基酸浓度高于正常组。这些结果表明，骨骼肌在麻醉期间体温变化中起重要作用，并且在肌肉萎缩状态下氨基酸对麻醉诱导体温过低的作用减弱。此外，麻醉期间静脉输注氨基酸会导致血浆胰岛素水平升高。
