Imoto Akinobu, Yokoyama Takeshi, Suwa Kunio, Yamasaki Fumiyasu, Yatabe Tomoaki, Yokoyama Reiko, Yamashita Koichi, Selldén Eva
Department of Anesthesiology and Critical Care Medicine, Kochi Medical School, Nankoku, Japan.
J Nutr Sci Vitaminol (Tokyo). 2010;56(2):104-8. doi: 10.3177/jnsv.56.104.
We hypothesized that, with oral or intestinal administration of amino acids (AA), we may reduce hypothermia during general anesthesia as effectively as with intravenous AA. We, therefore, examined the effect of bolus oral and continuous intestinal AA in preventing hypothermia in rats. Male Wistar rats were anesthetized with sevoflurane for induction and with propofol for maintenance. In the first experiment, 30 min before anesthesia, rats received one bolus 42 mL/kg of AA solution (100 g/L) or saline orally. Then for the next 3 h during anesthesia, they received 14 mL/kg/h of AA and/or saline intravenously. They were in 4 groups: I-A/A, both AA; I-A/S, oral AA and intravenous saline; I-S/A, oral saline and intravenous AA; I-S/S, both saline. In the second experiment, rats received 14 mL/kg/h duodenal AA and/or saline for 2 h. They were in 3 groups: II-A/S, duodenal AA and intravenous saline; II-S/A, duodenal saline and intravenous AA; II-S/S, both saline. Core body temperature was measured rectally. After the second experiment, serum electrolytes were examined. In both experiments, rectal temperature decreased in all groups during anesthesia. However, the decrease in rectal temperature was significantly less in groups receiving AA than in groups receiving only saline. In the second experiment, although there was no significant difference in the decrease in body temperature between II-A/S and II-S/A, Na(+) concentration was significantly lower in II-S/A. In conclusion, AA, administered orally or intestinally, tended to keep the body temperature stable during anesthesia without disturbing electrolyte balance. These results suggest that oral or enteral AA may be useful for prevention of hypothermia in patients.
我们假设，通过口服或肠道给予氨基酸（AA），我们可以像静脉输注氨基酸一样有效地降低全身麻醉期间的体温过低情况。因此，我们研究了一次性口服和持续肠道给予氨基酸对预防大鼠体温过低的效果。雄性Wistar大鼠用七氟醚诱导麻醉，丙泊酚维持麻醉。在第一个实验中，麻醉前30分钟，大鼠口服42 mL/kg的氨基酸溶液（100 g/L）或生理盐水。然后在接下来的3小时麻醉期间，它们静脉输注14 mL/kg/h的氨基酸和/或生理盐水。它们分为4组：I-A/A，均为氨基酸；I-A/S，口服氨基酸和静脉输注生理盐水；I-S/A，口服生理盐水和静脉输注氨基酸；I-S/S，均为生理盐水。在第二个实验中，大鼠十二指肠给予14 mL/kg/h的氨基酸和/或生理盐水，持续2小时。它们分为3组：II-A/S，十二指肠给予氨基酸和静脉输注生理盐水；II-S/A，十二指肠给予生理盐水和静脉输注氨基酸；II-S/S，均为生理盐水。通过直肠测量核心体温。第二个实验后，检测血清电解质。在两个实验中，所有组在麻醉期间直肠温度均下降。然而，接受氨基酸的组直肠温度下降明显少于仅接受生理盐水的组。在第二个实验中，尽管II-A/S和II-S/A之间体温下降没有显著差异，但II-S/A组的Na(+)浓度显著较低。总之，口服或肠道给予氨基酸在麻醉期间倾向于保持体温稳定，且不干扰电解质平衡。这些结果表明，口服或肠内给予氨基酸可能对预防患者体温过低有用。
