Ando Satoko, Kanazawa Masahiro, Tsuda Michio, Suzuki Toshiyasu
Department of Anesthesiology, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan.
J Nutr Sci Vitaminol (Tokyo). 2012;58(2):143-8. doi: 10.3177/jnsv.58.143.
A decrease in core temperature during general anesthesia is attenuated by infusion of an intravenous amino acid mixture. The purpose of the present study was to investigate the influence of physical and endocrine changes caused by ovariectomy on the inhibitory effect of amino acid infusion on anesthesia-induced hypothermia. Sprague-Dawley female rats were divided into a sham-operated (Sham) group and an ovariectomized (OVX) group. Saline solution or an amino acid mixture solution was infused for 180 min during sevoflurane anesthesia, and the rectal temperature was measured (4 groups). Intraperitoneal white adipose tissue mass, bilateral gastrocnemius weight and plasma insulin levels were measured. In the Sham rats, no inhibitory effect of the amino acid mixture on anesthesia-induced hypothermia was found, while in the OVX rats, hypothermia was significantly decreased. The intraperitoneal fat weight/body weight ratio was significantly higher in the OVX rats than in the Sham rats, but the gastrocnemius weight/body weight ratio was not significantly different. After amino acid infusion, the plasma insulin level was significantly higher in the OVX rats than in the Sham rats. In conclusion, our findings suggest that, in rats, ovarian function or female hormone affects protein turnover mediated by increase in insulin secretion and, thus, decreases the inhibitory effect of an infusion of amino acid mixture on anesthesia-induced hypothermia.
全身麻醉期间输注静脉氨基酸混合物可减轻核心体温的下降。本研究的目的是探讨卵巢切除术引起的身体和内分泌变化对氨基酸输注对麻醉诱导低温的抑制作用的影响。将Sprague-Dawley雌性大鼠分为假手术（Sham）组和卵巢切除（OVX）组。在七氟醚麻醉期间输注生理盐水或氨基酸混合溶液180分钟，并测量直肠温度（4组）。测量腹膜内白色脂肪组织质量、双侧腓肠肌重量和血浆胰岛素水平。在假手术大鼠中，未发现氨基酸混合物对麻醉诱导低温有抑制作用，而在卵巢切除大鼠中，低温显著降低。卵巢切除大鼠的腹膜内脂肪重量/体重比显著高于假手术大鼠，但腓肠肌重量/体重比无显著差异。输注氨基酸后，卵巢切除大鼠的血浆胰岛素水平显著高于假手术大鼠。总之，我们的研究结果表明，在大鼠中，卵巢功能或雌性激素通过增加胰岛素分泌影响蛋白质周转，从而降低氨基酸混合溶液输注对麻醉诱导低温的抑制作用。
