Life Sciences Institute and Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.
Autophagy. 2010 Jul;6(5):642-4. doi: 10.4161/auto.6.5.12092. Epub 2010 Jul 1.
The transparency, external development and simple drug administration of zebrafish embryos makes them a useful model for studying autophagy during embryonic development in vivo. Cloning of zebrafish lc3 and generation of a transgenic GFP-Lc3 fish line provide excellent tools to monitor autophagy in this organism. ( 1) This protocol discusses several convenient autophagy assays in zebrafish, including immunoblotting of Lc3 lipidation, microscopy imaging of GFP-Lc3 and lysosomal staining.
斑马鱼胚胎的透明性、体外发育和简单的给药方式使它们成为研究体内胚胎发育过程中自噬的有用模型。斑马鱼 lc3 的克隆和 GFP-Lc3 转基因鱼系的建立为监测该生物体内的自噬提供了极好的工具。(1)本方案讨论了几种在斑马鱼中进行自噬检测的方法,包括 LC3 脂质化的免疫印迹、GFP-Lc3 的显微镜成像和溶酶体染色。