Browne P V, Lawler M, O'Riordan J, Humphries P, McCann S R
Department of Haematology/Oncology, St James's Hospital, Dublin, Ireland.
Bone Marrow Transplant. 1991 Feb;7(2):167-9.
We report a case of acute lymphoblastic leukaemia relapsing after allogeneic bone marrow transplantation in which the polymerase chain reaction (PCR) was used to assess chimeric status. This technique demonstrated the progressive reappearance of host cells prior to clinical relapse. The relapse was of host cell origin as shown by the presence of female (recipient) metaphases containing an abnormal chromosomal marker (iso 9q) which had also been present at initial diagnosis. The emergence of host cells in this case, detected only by PCR techniques but not by cytogenetic methods, appeared to herald overt relapse. PCR analysis provides a sensitive tool for detecting a progressive rise in host cell numbers which may predict clinical relapse.
我们报告了1例急性淋巴细胞白血病患者，该患者在异基因骨髓移植后复发，我们使用聚合酶链反应（PCR）评估嵌合状态。这项技术显示，在临床复发之前，宿主细胞逐渐重新出现。复发源自宿主细胞，这一点通过含有异常染色体标记（9号染色体等臂）的女性（受体）中期分裂相得以证实，该标记在初次诊断时也已存在。在该病例中，宿主细胞的出现仅通过PCR技术检测到，而未通过细胞遗传学方法检测到，这似乎预示着明显的复发。PCR分析为检测宿主细胞数量的逐渐增加提供了一种灵敏的工具，这可能预示着临床复发。
