Chalmers E A, Sproul A M, Mills K I, Gibson B E, Burnett A K
Leukaemia Research Fund Laboratories, Glasgow Royal Infirmary, UK.
Bone Marrow Transplant. 1990 Dec;6(6):399-403.
Engraftment following allogeneic bone marrow transplantation (BMT) was assessed in three cases, two of which were sex-mismatched and one sex-matched. The polymerase chain reaction (PCR) was used to amplify the hypervariable region lying 3' to the apolipoprotein B gene on chromosome 2. Amplification of this region provided informative marker bands capable of distinguishing host/donor populations in each case. The method allowed rapid analysis of minimal numbers of cells from peripheral blood and bone marrow in the early stages following BMT and was predictive of either successful engraftment or graft failure.
对三例同种异体骨髓移植（BMT）后的植入情况进行了评估，其中两例为性别不匹配，一例为性别匹配。采用聚合酶链反应（PCR）扩增位于2号染色体载脂蛋白B基因3'端的高变区。该区域的扩增产生了信息性标记带，能够区分每个病例中的宿主/供体群体。该方法能够在BMT后的早期阶段快速分析来自外周血和骨髓的少量细胞，并可预测植入成功或移植失败。
