Department of Civil Engineering, Environmental Engineering Laboratory, University of Patras, 26500 Patras, Greece.
Environ Sci Technol. 2010 Jun 15;44(12):4539-44. doi: 10.1021/es100107a.
Bacteriophage MS2 and PhiX174 were used as surrogates for human viruses in order to investigate the interaction between viruses and clay particles. The selected phyllosilicate clays were kaolinite and bentonite (>90% montmorillonite). A series of static and dynamic experiments were conducted at two different temperatures (4 and 25 degrees C) to investigate the effect of temperature and agitation (dynamic experiments) on virus adsorption onto clays. Appropriate adsorption isotherms were determined. Electrokinetic features of bacteriophages and clays were quantified at different pH and ionic strength (IS). Moreover, interaction energies between viruses and clays were calculated for the experimental conditions (pH 7 and IS = 2 mM) by applying the DLVO theory. The experimental results shown that virus adsorption increases linearly with suspended virus concentration. The observed distribution coefficient (K(d)) was higher for MS2 than PhiX174. The observed K(d) values were higher for the dynamic than static experiments, and increased with temperature. The results of this study provided basic information for the effectiveness of clays to remove viruses by adsorption from dilute aqueous solutions. No previous study has explored the combined effect of temperature and agitation on virus adsorption onto clays.
噬菌体 MS2 和 PhiX174 被用作人类病毒的替代品，以研究病毒与粘土颗粒之间的相互作用。选择的层状硅酸盐粘土为高岭土和膨润土（>90%蒙脱石）。在两个不同温度（4 和 25°C）下进行了一系列静态和动态实验，以研究温度和搅拌（动态实验）对病毒吸附到粘土上的影响。确定了适当的吸附等温线。在不同 pH 和离子强度（IS）下定量了噬菌体和粘土的电动特性。此外，通过应用 DLVO 理论，针对实验条件（pH 7 和 IS = 2 mM）计算了病毒与粘土之间的相互作用能。实验结果表明，病毒吸附随悬浮病毒浓度呈线性增加。观察到的分配系数（Kd）对于 MS2 高于 PhiX174。与静态实验相比，动态实验中的观察到的 Kd 值更高，并且随温度升高而增加。本研究的结果为粘土通过从稀水溶液中吸附去除病毒的有效性提供了基本信息。以前没有研究探讨温度和搅拌对病毒吸附到粘土上的综合影响。
