Fernandez Oscar Orlando Araya, Pereira José Aires, Campos Fábio Guilherme, Araya Carolina Mardegan, Marinho Gabriele Escocia, Novo Rafaela de Souza, Oliveira Thais Silva de, Franceschi Yara Tinoco, Martinez Carlos Augusto Real
Laboratório de Investigação Médica do Programa de Pós-Graduação em Ciências da Saúde, Universidade São Francisco, Bragança Paulista, SP, Brasil.
Arq Bras Cir Dig. 2018 Aug 16;31(3):e1391. doi: 10.1590/0102-672020180001e1391.
The effects of topical application of sucralfate (SCF) on the tissue content of MUC-2 protein have not yet been evaluated in experimental models of diversion colitis.
To measure the tissue content of MUC-2 protein in the colonic mucosa diverted from fecal stream submitted to the SCF intervention.
Thirty-six rats underwent derivation of intestinal transit through proximal colostomy and distal mucous fistula. The animals were divided into three groups which were submitted application of enemas with saline, SCF 1 g/kg/day and SCF 2 g/kg/day. Each group was divided into two subgroups, according to euthanasia was done after two or four weeks. The colitis diagnosis was established by histopathological study and the inflammatory intensity was evaluated by previously validated scale. The MUC-2 protein was identified by immunohistochemistry and the tissue content was measured computerized morphometry).
The application of enemas with SCF in the concentration of 2 g/kg/day reduced inflammatory score of the segments that were diverted from fecal stream. The content of MUC-2 in diverted colon of the animals submitted to the intervention with SCF, independently of intervention period and the used concentration, was significantly greater than animals submitted to the application of enemas containing saline (p< 0.01). The content of MUC-2 after the intervention with SCF in the concentration of 2 g/kg/day was significantly higher when compared to the animals submitted to the application containing SCF at concentration of 1.0 g/kg/day (p<0.01). The tissue content of MUC-2 reached the highest values after intervention with SCF in the concentration of 2 g/kg/day for four weeks (p<0.01).
The preventive application of enemas containing SCF reduces the inflammatory score and avoids the reduction of tissue content of MUC-2, suggesting that the substance is a valid therapeutic strategy to preserve the mucus layer that covers the intestinal epithelium.
在转流性结肠炎实验模型中，尚未评估局部应用硫糖铝（SCF）对MUC-2蛋白组织含量的影响。
测量接受SCF干预的粪便转流结肠黏膜中MUC-2蛋白的组织含量。
36只大鼠通过近端结肠造口术和远端黏液瘘进行肠道转流。将动物分为三组，分别给予生理盐水灌肠、1 g/kg/天的SCF灌肠和2 g/kg/天的SCF灌肠。根据在两周或四周后实施安乐死，每组再分为两个亚组。通过组织病理学研究确立结肠炎诊断，并使用先前验证的量表评估炎症强度。通过免疫组织化学鉴定MUC-2蛋白，并通过计算机形态测量法测量组织含量。
每天给予2 g/kg浓度的SCF灌肠可降低粪便转流节段的炎症评分。接受SCF干预的动物，无论干预时间和使用浓度如何，其转流结肠中MUC-2的含量均显著高于接受生理盐水灌肠的动物（p<0.01）。与接受1.0 g/kg浓度SCF灌肠的动物相比，接受2 g/kg/天浓度SCF干预后MUC-2的含量显著更高（p<0.01）。在接受2 g/kg/天浓度的SCF干预四周后，MUC-2的组织含量达到最高值（p<0.01）。
预防性应用含SCF的灌肠剂可降低炎症评分，并避免MUC-2组织含量的减少，表明该物质是一种有效的治疗策略，可保护覆盖肠上皮的黏液层。
