[焦磷酸测序法检测猪流感病毒中金刚烷胺耐药性的分子标志物]
[Detection of molecular markers of amantadine resistance in swine influenza viruses by pyrosequencing].
作者信息
Liu Hualei, Lv Yan, Huang Weijian, Yan Minghua, Zhang Wei, Li Mingyi, Wang Qinghua, Li Jinming, Zheng Dongxia, Zhao Yunling, Sun Chengying, Wang Zhiliang
机构信息
China Animal Health and Epidemiology Center, Qingdao 266032, China.
出版信息
Wei Sheng Wu Xue Bao. 2010 Mar;50(3):395-9.
OBJECTIVE
To evaluate the resistance against the adamantine of multi-genotype (H1N1, H3N2 and H9N2) swine influenza viruses isolated from China in recent years by pyrosequencing.
METHODS
Mutation in one of five key amino acid residues (positions 26, 27, 30, 31 and 34) within the M2 protein of influenza A viruses, leading to resistance against the adamantine class of anti-influenza drugs. The residues L26, V27, A30, S31, and G34 in the M2 protein were targeted for pyrosequencing,and 10 swine influenza viruses obtained from China during 2004 to 2008 were used to perform the amantadine resistance analysis.
RESULTS
All 5 H1N1 swine influenza viruses were adamantine resistance, three mutations were founded in these isolates, namely V27T, V27I and S31N. Other five isolates, including four H3N2 and one H9N2 swine influenza virus, were proved to be sensitive to amantadine.
CONCLUSION
Pyrosequencing technology based on the M2 gene can be used to determine the amantadine resistance for multi-genotype swine influenza viruses.
目的
通过焦磷酸测序技术评估近年来从中国分离出的多基因型（H1N1、H3N2和H9N2）猪流感病毒对金刚烷类药物的耐药性。
方法
甲型流感病毒M2蛋白五个关键氨基酸残基（第26、27、30、31和34位）之一发生突变会导致对金刚烷类抗流感药物产生耐药性。以M2蛋白中的L26、V27、A30、S31和G34残基为焦磷酸测序靶点，使用2004年至2008年期间从中国获得的10株猪流感病毒进行金刚烷胺耐药性分析。
结果
所有5株H1N1猪流感病毒均对金刚烷类药物耐药，在这些分离株中发现了3种突变，即V27T、V27I和S31N。其他5株分离株，包括4株H3N2和1株H9N2猪流感病毒，被证明对金刚烷胺敏感。
结论
基于M2基因的焦磷酸测序技术可用于检测多基因型猪流感病毒对金刚烷胺的耐药性。
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