Marsal J, Solsona C, Rabasseda X, Blasi J, Casanova A
Departament de Biologia Cel.lular i Anatomic Patològica, Facultat de Medicina, Hospital de Bellvitge, Universitat de Barcelona, Barcelona, Spain.
Neurochem Int. 1987;10(3):295-302. doi: 10.1016/0197-0186(87)90103-3.
We report here the effects of Botulinum Toxin type A on the release of ATP and Acetylcholine from Torpedo electric organ synaptosomes. Our results show that Botulinum Toxin type A inhibits specifically the K(+)-induced release of Acetylcholine from synaptosomes without affecting the release of ATP. Membrane potential and calcium uptake into cholinergic nerve terminals are not modified after Botulinum Toxin poisoning. It is suggested that either most of the ATP released during the depolarization of the cholinergic synaptosomes does not originate from cholinergic synaptic vesicles or that there are two populations of synaptic vesicles, Acetylcholine-enriched synaptic vesicles and ATP-enriched synaptic vesicles. However, the possibility that the ACh and ATP released could come from different intrasynaptosomal compartments cannot be excluded.
我们在此报告A型肉毒杆菌毒素对电鳐电器官突触体中ATP和乙酰胆碱释放的影响。我们的结果表明，A型肉毒杆菌毒素特异性抑制K(+)诱导的突触体中乙酰胆碱的释放，而不影响ATP的释放。肉毒杆菌毒素中毒后，膜电位和钙摄取进入胆碱能神经末梢没有改变。这表明，要么胆碱能突触体去极化过程中释放的大部分ATP并非来自胆碱能突触小泡，要么存在两种突触小泡群体，即富含乙酰胆碱的突触小泡和富含ATP的突触小泡。然而，乙酰胆碱和ATP的释放可能来自不同突触体内隔室的可能性也不能排除。
