Malminen O, Kontro P
University of Tampere, Department of Biomedical Sciences, P.O.B. 607, SF-33101 Tampere, Finland.
Neurochem Int. 1987;11(1):113-7. doi: 10.1016/0197-0186(87)90157-4.
The actions of taurine on the solubilized GABA-benzodiazepine receptor complex were investigated, and the results compared to those obtained with detergent-treated membrane-bound receptors. The receptor complex of adult rat brain was solubilized with Triton X-100 or CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulphonate). The properties of the solubilized GABA and flunitrazepam binding sites were similar to those in washed brain membranes. Taurine displaced GABA from its receptor sites and inhibited GABA stimulation of flunitrazepam binding to receptor complexes solubilized with Triton X-100. Thus the modulatory action of taurine on the receptor complex in washed membrane preparations was well preserved after this solubilization. No specific taurine binding to either Triton- or CHAPS-solubilized sample could be demonstrated.
研究了牛磺酸对溶解的γ-氨基丁酸-苯二氮䓬受体复合物的作用，并将结果与用去污剂处理的膜结合受体的结果进行比较。用曲拉通X-100或3-[(3-胆酰胺丙基)二甲基铵]-1-丙烷磺酸盐（CHAPS）溶解成年大鼠脑的受体复合物。溶解的γ-氨基丁酸和氟硝西泮结合位点的特性与洗涤过的脑膜中的相似。牛磺酸从其受体位点取代γ-氨基丁酸，并抑制γ-氨基丁酸对氟硝西泮与用曲拉通X-100溶解的受体复合物结合的刺激作用。因此，在这种溶解后，牛磺酸对洗涤过的膜制剂中受体复合物的调节作用得到了很好的保留。未证明牛磺酸与曲拉通或CHAPS溶解的样品有特异性结合。
