Department of Social and Behavioral Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
Am J Physiol Gastrointest Liver Physiol. 2009 Nov;297(5):G1028-34. doi: 10.1152/ajpgi.90692.2008.
Since no previous studies have reported dual measurements of stomach and duodenal motility in conscious mice, we developed a manometric method to measure the gastroduodenal motility in the physiological fed and fasted states of conscious mice. By this method we measured, for the first time, the gastroduodenal motility in Y2 knockout mice and analyzed the effects of ghrelin on the gastroduodenal motility in conscious mice. To evaluate this new method, we provide the comparison on the effects of CCK-8 examined by present and previous methods. In the fasted state of mice, phase III-like contractions with frequencies of 7.8 +/- 0.5 contractions/h in the antrum and 6.6 +/- 0.7 contractions/h in the duodenum were observed. This fasted pattern was disrupted and replaced by the fed pattern after feeding, with an increase of the motor index (MI) immediately after feeding. Intravenous injection of ghrelin induced the fasted pattern in the duodenum when injected in the fed state and increased %MI (114.3 +/- 9.8%) compared with saline-injected controls (64.4 +/- 9.6%) in the antrum. Intravenous injection of CCK-8 disrupted phase III-like contractions in both antrum and duodenum, which were replaced by fed-like motor patterns accompanied with the elevation of baseline pressure. In Y2 knockout mice, the frequency of phase III-like contractions was decreased in the antrum compared with wild-type mice and the immediate increase of MI after feeding seen in wild-type mice was disrupted in Y2 knockout mice. Our model provides a new method for studies of gastrointestinal motility in various mouse models, including transgenic and knockout ones.
由于以前的研究没有报道过在清醒状态下的小鼠中胃和十二指肠运动的双重测量，因此我们开发了一种测压方法来测量清醒状态下的胃十二指肠运动。通过这种方法，我们首次测量了 Y2 敲除小鼠的胃十二指肠运动，并分析了生长激素释放肽对清醒状态下小鼠胃十二指肠运动的影响。为了评估这种新方法，我们提供了目前方法和以前方法对胆囊收缩素 8 影响的比较。在禁食状态下，胃窦和十二指肠的 III 相样收缩的频率分别为 7.8 +/- 0.5 收缩/小时和 6.6 +/- 0.7 收缩/小时。这种禁食模式在进食后被打乱并被进食模式所取代，进食后立即增加运动指数（MI）。在进食状态下静脉注射生长激素释放肽会在十二指肠中引起禁食模式，并使 MI（114.3 +/- 9.8％）与盐水对照（64.4 +/- 9.6％）相比显著增加。静脉注射胆囊收缩素 8 会破坏胃窦和十二指肠中的 III 相样收缩，取而代之的是伴随基线压力升高的进食样运动模式。在 Y2 敲除小鼠中，与野生型小鼠相比，胃窦中的 III 相样收缩频率降低，而在野生型小鼠中观察到的进食后 MI 的立即增加在 Y2 敲除小鼠中被破坏。我们的模型为各种小鼠模型（包括转基因和敲除模型）的胃肠道运动研究提供了一种新方法。
