Instituto Valenciano de Infertilidad (IVI), University of Valencia, Plaza de la Policía Local, 3, 46015 Valencia, Spain.
Hum Reprod. 2010 Jul;25(7):1713-21. doi: 10.1093/humrep/deq098. Epub 2010 May 24.
The aim of the present study was to evaluate the implication of male factor, in terms of sperm DNA oxidation and fragmentation, and Y chromosome microdeletions in recurrent spontaneous abortion (RSA) of unknown origin in a strictly selected cohort.
A prospective cohort study was carried out in a private university-affiliated setting. Three groups, each comprised of 30 males, were compared. The first was formed by healthy and fertile sperm donors (SD) with normal sperm parameters (control group), the second by men presenting severe oligozoospermia (SO) without RSA history, and the third by men from couples who had experienced idiopathic RSA. Frequency of Y chromosome microdeletions and mean sperm DNA fragmentation and oxidation were determined.
Y chromosome microdeletions were not detected in any of the males enrolled in the study. Moreover, sperm DNA oxidation measurements were not demonstrated to be relevant to RSA. Interestingly, sperm DNA fragmentation was higher in the SO group than in the RSA and the SD groups, and also higher in the RSA group compared with the SD group, but lacked an adequate predictive power to be employed as a discriminative test of RSA condition.
Sperm DNA features and Y chromosome microdeletions do not seem to be related to RSA of unknown origin. Other molecular features of sperm should be studied to determine their possible influence on RSA. Clinicaltrials.gov reference: NCT00447395.
本研究旨在评估精子 DNA 氧化和碎片化以及 Y 染色体微缺失在不明原因复发性自然流产（RSA）中的意义，研究对象为严格选择的队列中的男性。
在一家私立大学附属医院进行前瞻性队列研究。比较了三个组，每组 30 名男性。第一组由具有正常精子参数的健康和有生育能力的精子供体（SD）组成（对照组），第二组由无 RSA 病史的严重少精症（SO）男性组成，第三组由经历特发性 RSA 的夫妇的男性组成。确定 Y 染色体微缺失的频率以及平均精子 DNA 碎片化和氧化程度。
研究中未发现任何男性存在 Y 染色体微缺失。此外，精子 DNA 氧化测量与 RSA 无关。有趣的是，SO 组的精子 DNA 碎片化程度高于 RSA 组和 SD 组，而 RSA 组又高于 SD 组，但缺乏足够的预测能力作为 RSA 状况的鉴别试验。
精子 DNA 特征和 Y 染色体微缺失似乎与不明原因的 RSA 无关。应研究精子的其他分子特征，以确定它们对 RSA 的可能影响。Clinicaltrials.gov 注册号：NCT00447395。
