来自黑指纹海兔神经系统的含丙酮酸化半乳糖的新型膦酰糖鞘脂。

Novel phosphonoglycosphingolipids containing pyruvylated galactose from the nervous system of Aplysia kurodai.

作者信息

Araki S, Abe S, Satake M, Hayashi A, Kon K, Ando S

机构信息

Department of Neurochemistry, Niigata University, Japan.

出版信息

Eur J Biochem. 1991 Jun 15;198(3):689-95. doi: 10.1111/j.1432-1033.1991.tb16068.x.

DOI:10.1111/j.1432-1033.1991.tb16068.x

PMID:2050147

Abstract

We have reported the existence of a phosphonoglycosphingolipid containing a pyruvylated galactose, FGL-IIb, in nerve fibers of Aplysia kurodai (Araki, S., Abe, S., Ando, S., Kon, K., Fujiwara, N. & Satake, M. (1989) J. Biol. Chem. 264, 19922-19927). We have now isolated two other pyruvylated galactose-containing phosphonoglycosphingolipids, named FGL-V and FGL-IIa, from the nervous tissue of Aplysia, and characterized them as [3,4-O-(S-1-carboxyethylidene)]Gal beta 1----3GalNAc alpha 1----3[6'-O-(2- aminoethylphosphonyl)Gal alpha 1----2] (2-aminoethylphosphonyl----6) Gal beta 1----4Glc beta 1----1 ceramide and [3,4,O-(S-1-carboxyethylidene)] Gal beta 1----3GalNAc alpha 1----3[6'-O-(2-aminoethylphosphonyl)Gal alpha 1----2]Gal beta 1----4Glc beta 11----ceramide, respectively. Their major aliphatic components are palmitic acid, octadeca-4-sphingenine and anteisononadeca-4-sphingenine. Thus, the nervous system of Aplysia contains several pyruvylated phosphonoglycolipids.

摘要

我们已经报道了在日本黑兔的神经纤维中存在一种含有丙酮酸化半乳糖的膦糖鞘脂，FGL-IIb（荒木，S.，阿部，S.，安藤，S.，孔，K.，藤原，N.和里竹，M.（1989年）《生物化学杂志》264，19922 - 19927）。我们现在从日本黑兔的神经组织中分离出另外两种含有丙酮酸化半乳糖的膦糖鞘脂，命名为FGL-V和FGL-IIa，并将它们分别鉴定为[3,4 - O -（S - 1 - 羧基亚乙基）]Galβ1----3GalNAcα1----3[6'-O -（2 - 氨基乙基膦酰基）Galα1----2]（2 - 氨基乙基膦酰基----6）Galβ1----4Glcβ1----1神经酰胺和[3,4,O -（S - 1 - 羧基亚乙基）]Galβ1----3GalNAcα1----3[6'-O -（2 - 氨基乙基膦酰基）Galα1----2]Galβ1----4Glcβ11----神经酰胺。它们的主要脂肪族成分是棕榈酸、十八碳-4 - 鞘氨醇和反异十九碳-4 - 鞘氨醇。因此，日本黑兔的神经系统含有几种丙酮酸化的膦糖脂。