Araki S, Abe S, Yamada S, Satake M, Fujiwara N, Kon K, Ando S
Department of Neurochemistry, Faculty of Medicine, Niigata University.
J Biochem. 1992 Oct;112(4):461-9. doi: 10.1093/oxfordjournals.jbchem.a123922.
Two novel acidic glycosphingolipids containing pyruvylated galactose were purified from the nervous tissue of Aplysia kurodai by successive Iatrobeads column chromatographies. By component analysis, sugar analysis, permethylation studies, fast atom bombardment-mass spectrometry, and proton magnetic resonance spectrometry, the structures of these acidic glycosphingolipids, named F-9 and FGL-I, were determined to be: [3,4-O-(S-1-carboxyethylidene)]Gal beta 1-->3 GalNAc alpha 1-->3[6'-O-(2-aminoethylphosphonyl)Gal alpha 1-->2] (2-aminoethylphosphoryl 1-->6)Gal beta 1-->4Glc beta 1-->1ceramide and [3,4-O-(S-1-carboxyethylidene)] Gal beta 1-->3GalNAc alpha 1-->3(Fuc alpha 1-->2)(2-aminoethylphosphonyl-->6 Gal beta 1-->4Glc beta 1-->1ceramide, octadeca-4-sphingenine and anteisononadeca-4-sphingenine. Thus, pyruvylated glycosphingolipids containing phosphoethanolamine in addition to or in place of 2-aminoethylphosphonate are present in the nervous system of Aplysia.
通过连续的离子交换树脂柱色谱法，从黑背海兔的神经组织中纯化出两种含有丙酮酸化半乳糖的新型酸性糖鞘脂。通过成分分析、糖类分析、全甲基化研究、快原子轰击质谱法和质子磁共振光谱法，确定了这两种名为F-9和FGL-I的酸性糖鞘脂的结构为：[3,4-O-(S-1-羧基亚乙基)]半乳糖β1→3 N-乙酰半乳糖胺α1→36'-O-(2-氨基乙基膦酰基)半乳糖α1→2半乳糖β1→4葡萄糖β1→1神经酰胺和[3,4-O-(S-1-羧基亚乙基)]半乳糖β1→3 N-乙酰半乳糖胺α1→3(岩藻糖α1→2)(2-氨基乙基膦酰基→6半乳糖β1→4葡萄糖β1→1神经酰胺、十八碳-4-鞘氨醇和反式十九碳-4-鞘氨醇。因此，在黑背海兔的神经系统中存在除2-氨基乙基膦酸酯之外或取代它的含有磷酸乙醇胺的丙酮酸化糖鞘脂。
