Yarbrough J, Cunningham M, Yamanaka H, Thurman R, Badr M
University of Missouri-Kansas City, Kansas City 64108.
Hepatology. 1991 Jun;13(6):1229-34.
Liver regeneration after partial hepatectomy is accompanied by altered hepatic intermediary metabolism. Because the organochlorine compound mirex also causes liver cell growth, the purpose of this study was to investigate hepatic carbohydrate and oxygen metabolism in perfused livers from mirex-treated rats and to localize cell proliferation in this model. Pretreatment with mirex (100 mg/kg, intragastrically) increased liver/body weight ratios and DNA synthesis in livers of fed rats, effects that were markedly diminished in livers of fasted rats. This finding shows that liver growth caused by mirex, as is the case after partial hepatectomy, is hindered when animals are deprived of food. Furthermore, perfused livers from mirex-treated rats had depleted glycogen stores but significantly elevated oxygen uptake compared with livers from control rats. Increases in oxygen uptake and hepatocellular proliferation were observed mostly in periportal regions of the liver lobule. In regenerating livers, most DNA synthesis was reported to also occur in these regions of the liver lobule. Taken together, these data show that liver cell growth caused by mirex is accompanied by changes in hepatic intermediary metabolism and sublobular proliferation similar to those observed after partial hepatectomy.
部分肝切除术后的肝脏再生伴随着肝脏中间代谢的改变。由于有机氯化合物灭蚁灵也会引起肝细胞生长，本研究的目的是调查灭蚁灵处理大鼠的灌注肝脏中的肝碳水化合物和氧代谢，并在此模型中定位细胞增殖。用灭蚁灵（100mg/kg，灌胃）预处理可增加喂食大鼠肝脏的肝/体重比和DNA合成，而禁食大鼠肝脏中的这些效应则明显减弱。这一发现表明，与部分肝切除术后的情况一样，当动物禁食时，灭蚁灵引起的肝脏生长会受到阻碍。此外，与对照大鼠的肝脏相比，灭蚁灵处理大鼠的灌注肝脏糖原储备耗尽，但氧摄取显著增加。氧摄取增加和肝细胞增殖主要发生在肝小叶的门周区域。据报道，在再生肝脏中，大多数DNA合成也发生在肝小叶的这些区域。综上所述，这些数据表明，灭蚁灵引起的肝细胞生长伴随着肝脏中间代谢的变化和小叶下增殖，类似于部分肝切除术后观察到的情况。
