Garcia-Gil M, Tongiorgi E, Cattani M, Cipollini G, Brunelli M
Department of Physiology and Biochemistry, University of Pisa, Italy.
Neurochem Int. 1989;15(2):161-8. doi: 10.1016/0197-0186(89)90096-x.
Incubation of leech segmental ganglia with [(32)P]phosphoric acid results in the labeling of 30 phosphoproteins, detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by autoradiography. Treatment with 8-bromo-cAMP or phosphodiesterase inhibitors induces a rise in the label of the following phosphoproteins: 96, 79, 63, 50, 40, 32, 28, 24, 23 and 20 kDa. One of the most intersting membrane-bound proteins studied has an apparent molecular weight of 79 kDa. It seems to be specific for the nervous tissue and inside the nervous system, for segmental ganglia. The role of these phosphoproteins in the mechanisms of learning processes is discussed.
用[（32）P]磷酸孵育水蛭节段神经节，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，随后进行放射自显影，可检测到30种磷蛋白被标记。用8-溴环磷酸腺苷或磷酸二酯酶抑制剂处理会导致以下磷蛋白的标记增加：96、79、63、50、40、32、28、24、23和20 kDa。所研究的最有趣的膜结合蛋白之一的表观分子量为79 kDa。它似乎对神经组织具有特异性，在神经系统内部，对节段神经节也是如此。本文讨论了这些磷蛋白在学习过程机制中的作用。
