急性转化逆转录病毒表达 Nras 来源于 HT-1080 纤维肉瘤细胞感染人类逆转录病毒 XMRV。
Acutely transforming retrovirus expressing Nras generated from HT-1080 fibrosarcoma cells infected with the human retrovirus XMRV.
机构信息
Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024, USA.
出版信息
J Virol. 2010 Aug;84(15):7908-10. doi: 10.1128/JVI.00389-10. Epub 2010 May 26.
Abstract
Virus from HT-1080 fibrosarcoma cells infected with the human retrovirus XMRV (xenotropic murine leukemia virus-related virus) can induce rare foci of transformation in rat 208F fibroblasts. Characterization of three such foci revealed that one produced an acutely transforming virus at a high titer. The virus consists of a mutant Nras cDNA from the HT-1080 cells inserted into a retroviral vector (added to the HT-1080 cells as a marker for infection) in place of internal vector sequences. These results show that XMRV can generate acutely transforming viruses at a low rate, as is typical of other replication-competent retroviruses, and reveal the potential for transforming virus contamination of retroviral vectors made from transformed cell lines.
摘要
源自 HT-1080 纤维肉瘤细胞并感染人类逆转录病毒 XMRV（异嗜性鼠白血病病毒相关病毒）的病毒可诱发大鼠 208F 成纤维细胞中罕见的转化灶。对其中三个转化灶的特征分析表明，其中一个产生了高滴度的急性转化病毒。该病毒由 HT-1080 细胞中的突变 Nras cDNA 插入逆转录病毒载体（作为感染的标记物添加到 HT-1080 细胞中）取代内部载体序列构成。这些结果表明，XMRV 能够以低频率产生急性转化病毒，这与其他复制型逆转录病毒相似，并揭示了源自转化细胞系的逆转录病毒载体存在转化病毒污染的可能性。
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