XMRV 逆转录病毒的左半部分存在于 NIH/3T3 瑞士鼠细胞的内源性逆转录病毒中。
The left half of the XMRV retrovirus is present in an endogenous retrovirus of NIH/3T3 Swiss mouse cells.
机构信息
Human Biology Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Seattle, WA 98109-1024, USA.
出版信息
J Virol. 2011 Sep;85(17):9247-8. doi: 10.1128/JVI.05137-11. Epub 2011 Jun 22.
Abstract
Xenotropic murine leukemia virus-related virus (XMRV) is a gammaretrovirus found in association with human prostate cancer and chronic fatigue syndrome, although these associations are controversial. XMRV shows at most 94% identity to known mouse retroviruses. Here we used XMRV-specific PCR to search for a more closely related source of XMRV in mice. While we could not find a complete copy, we did find a 3,600-bp region of XMRV in an endogenous retrovirus present in NIH/3T3 cells. These results show that XMRV has clear ancestors in mice and highlight another possible source of contamination in PCR assays for XMRV.
摘要
Xenotropic murine leukemia virus-related virus (XMRV) 是一种与人类前列腺癌和慢性疲劳综合征相关的γ逆转录病毒，尽管这些关联存在争议。XMRV 与已知的鼠逆转录病毒的相似度最高可达 94%。在这里，我们使用 XMRV 特异性 PCR 来搜索与 XMRV 更密切相关的鼠源。虽然我们未能找到完整的拷贝，但我们确实在 NIH/3T3 细胞中发现了一种内源性逆转录病毒中的 XMRV 3600bp 片段。这些结果表明 XMRV 在鼠中有明确的祖先，这突显了 XMRV PCR 检测中另一种可能的污染来源。
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本文引用的文献
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Science. 2011 Jul 1;333(6038):97-101. doi: 10.1126/science.1205292. Epub 2011 May 31.
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MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.MEGA5：用于最大似然法、进化距离法和最大简约法的分子进化遗传学分析。
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