Schrader S M, Turner T W, Simon S D
National Institute for Occupational Safety and Health, Division of Biomedical and Behavioral Science, Cincinnati, Ohio 45226-1988.
J Androl. 1991 Mar-Apr;12(2):126-31.
As part of a longitudinal study of human semen characteristics of unexposed workers, sperm motility measurements were made using computer-assisted sperm analysis. Motility analyses were conducted on monthly samples from 46 men for 9 months. Measurements of curvilinear velocity, straight-line velocity (VSL), linearity, amplitude of lateral head displacement (ALH), and beat-cross frequency were collected in eight microscope fields for each semen sample. The variability within a sample, between samples from the same individual (between monthly samples), and between individuals were calculated using a nested analysis of variance. For all sperm motility measurements, at least 90% of the variation was observed between cells within a semen sample. For all variables, the component of variation between subjects was the smallest percentage (ranging from 1.3% for ALH to 4.0% for VSL). When sample means were used in the nested analysis of variation, at least 75% of the variation was observed between samples from the same individual. These results will be useful in power calculations for future studies.
作为对未接触有害物质工人的人类精液特征进行纵向研究的一部分，使用计算机辅助精子分析对精子活力进行测量。对46名男性的月度样本进行了9个月的活力分析。每个精液样本在八个显微镜视野中收集曲线速度、直线速度（VSL）、线性度、头部侧向位移幅度（ALH）和鞭打交叉频率的测量值。使用嵌套方差分析计算样本内、同一个体的样本间（月度样本间）以及个体间的变异性。对于所有精子活力测量，在精液样本内的细胞间观察到至少90%的变异。对于所有变量，受试者间变异成分所占百分比最小（从ALH的1.3%到VSL的4.0%不等）。当在嵌套方差分析中使用样本均值时，在同一个体的样本间观察到至少75%的变异。这些结果将有助于未来研究的功效计算。
