Department of Plant Sciences, Laboratory of Plant Breeding, Wageningen University, PO Box 386, 6700 AJ, Wageningen, The Netherlands.
Mol Plant Pathol. 2006 Nov;7(6):499-510. doi: 10.1111/j.1364-3703.2006.00355.x.
SUMMARY We adapted and optimized the use of the Agrobacterium tumefaciens binary PVX expression system (PVX agroinfection) to screen Solanum plants for response to pathogen elicitors and applied the assay to identify a total of 11 clones of Solanum huancabambense and Solanum microdontum, out of 31 species tested, that respond to the elicitins INF1, INF2A and INF2B of Phytophthora infestans. Prior to this study, response to INF elicitins was only known in Nicotiana spp. within the Solanaceae. The identified S. huancabambense and S. microdontum clones also exhibited hypersensitivity-like cell death following infiltration with purified recombinant INF1, INF2A and INF2B, thereby validating the screening protocol. Comparison of INF elicitin activity revealed that Nicotiana plants responded to significantly lower concentrations than Solanum, suggesting variable levels of sensitivity to INF elicitins. We exploited natural variation in response to INF elicitins in the identified Solanum accessions to evaluate the relationship between INF recognition and late blight resistance. Interestingly, several INF-responsive Solanum plants were susceptible to P. infestans. Also, an S. microdontum xSolanum tuberosum (potato) population that segregates for INF response was generated but failed to identify a measurable contribution of INF response to resistance. These results suggest that in Solanum, INF elicitins are recognized as general elicitors and do not have a measurable contribution to disease resistance.
摘要 我们对根癌农杆菌二元 PVX 表达系统（PVX 农杆菌浸染）的使用进行了改良和优化，用于筛选对病原体诱导子有反应的茄属植物，并将该方法应用于鉴定总共 11 株对疫霉菌 INF1、INF2A 和 INF2B 诱导子有反应的胡安娜巴姆本塞姆茄和微齿茄克隆，在测试的 31 个物种中，仅有茄属植物对此类诱导子有反应。在此之前，在茄科植物中仅在茄属植物中发现对 INF 诱导子的反应。鉴定出的胡安娜巴姆本塞姆茄和微齿茄克隆在浸润纯化的重组 INF1、INF2A 和 INF2B 后也表现出类似于过敏反应的细胞死亡，从而验证了筛选方案。对 INF 诱导子活性的比较表明，与茄属植物相比，烟草植物对 INF 诱导子的反应浓度要低得多，这表明对 INF 诱导子的敏感性存在差异。我们利用鉴定出的茄属植物对 INF 诱导子的自然变异来评估 INF 识别与晚疫病抗性之间的关系。有趣的是，一些对 INF 诱导子有反应的茄属植物对疫霉菌易感。此外，还生成了一个对 INF 反应分离的微齿茄 x 马铃薯（土豆）群体，但未能鉴定出 INF 反应对抗性的可测量贡献。这些结果表明，在茄属植物中，INF 诱导子被识别为一般诱导子，对疾病抗性没有可测量的贡献。
