ATAC 和 Mediator 共激活因子形成一个稳定的复合物，并调节一组非编码 RNA 基因。
ATAC and Mediator coactivators form a stable complex and regulate a set of non-coding RNA genes.
机构信息
Department of Functional Genomics, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Parc d'Innovation, 1 rue Laurent Fries, CNRS UMR 7104, INSERM U 964, Université de Strasbourg, BP 10142, Illkirch Cedex 67404, France.
出版信息
EMBO Rep. 2010 Jul;11(7):541-7. doi: 10.1038/embor.2010.75. Epub 2010 May 28.
Abstract
The Ada-Two-A-containing (ATAC) histone acetyltransferase and Mediator coactivator complexes regulate independent and distinct steps during transcription initiation and elongation. Here, we report the identification of a new stable molecular assembly formed between the ATAC and Mediator complexes in mouse embryonic stem cells. Moreover, we identify leucine zipper motif-containing protein 1 as a subunit of this meta-coactivator complex (MECO). Finally, we demonstrate that the MECO regulates a subset of RNA polymerase II-transcribed non-coding RNA genes. Our findings establish that transcription coactivator complexes can form stable subcomplexes to facilitate their combined actions on specific target genes.
摘要
Ada-Two-A 内含（ATAC）组蛋白乙酰转移酶和中介体共激活复合物在转录起始和延伸过程中调节独立且不同的步骤。在这里，我们报告了在小鼠胚胎干细胞中鉴定出 ATAC 和中介体复合物之间形成的一种新的稳定的分子组装。此外，我们鉴定出亮氨酸拉链模体蛋白 1 是这个元共激活复合物（MECO）的一个亚基。最后，我们证明 MECO 调节一组 RNA 聚合酶 II 转录的非编码 RNA 基因。我们的研究结果表明，转录共激活复合物可以形成稳定的亚复合物，以促进它们在特定靶基因上的联合作用。
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