Abu Fanas S H, Drucker D B, Hull P S
Department of Restorative Dentistry, Turner Dental School, University of Manchester, UK.
J Dent. 1991 Apr;19(2):92-6. doi: 10.1016/0300-5712(91)90096-h.
The in vitro release of amoxycillin with clavulanic acid from acrylic strips at initial concentrations of 30, 40 and 50 per cent w/w was monitored using a double-beam ultraviolet spectrophotometer and compared with release of tetracycline hydrochloride. Highest levels of the antibacterial agents were released during the first 24 h period. Therapeutic levels of the drugs continued to be released during the subsequent 9 day period and were shown to be biologically active. Furthermore, for amoxycillin with clavulanic acid, an initial concentration of 40 per cent gave the highest level of release on day 10; while, for tetracycline, 50 per cent provided the highest level of release. Local application of 40 per cent amoxycillin with clavulanic acid incorporated into acrylic strips placed in periodontal pockets in patients with established periodontitis produced a marked change in the subgingival microflora as monitored by dark-field microscopy and cultural techniques. These changes in the subgingival flora were concomitant with elimination of bleeding on probing at the treated sites and were still evident 3 weeks after removal of the acrylic strips. The sensitivity of Bacteroides gingivalis (syn. Porphyromonas gingivalis) and Bacteroides intermedius (syn. Prevotella intermedia) isolated before and after treatment to amoxycillin with clavulanic acid remained unchanged.
使用双光束紫外分光光度计监测阿莫西林克拉维酸在初始浓度为30%、40%和50%（w/w）的丙烯酸酯条中的体外释放情况，并与盐酸四环素的释放情况进行比较。抗菌剂在最初24小时内释放量最高。在随后的9天内，药物的治疗水平持续释放，并显示具有生物活性。此外，对于阿莫西林克拉维酸，40%的初始浓度在第10天释放量最高；而对于四环素，50%的初始浓度释放量最高。将含40%阿莫西林克拉维酸的丙烯酸酯条局部应用于患有中度牙周炎患者的牙周袋中，通过暗视野显微镜检查和培养技术监测发现，龈下微生物群发生了显著变化。龈下菌群的这些变化伴随着治疗部位探诊出血的消除，并且在移除丙烯酸酯条3周后仍然明显。治疗前后分离出的牙龈拟杆菌（同义词：牙龈卟啉单胞菌）和中间拟杆菌（同义词：中间普雷沃菌）对阿莫西林克拉维酸的敏感性保持不变。
