Institute of Biological and Environmental Science, University of Aberdeen, Aberdeen, AB24 2TZ, UK.
Comp Biochem Physiol A Mol Integr Physiol. 2011 Mar;158(3):323-8. doi: 10.1016/j.cbpa.2010.05.012. Epub 2010 May 25.
The (13)C-labelled Na-bicarbonate technique uses stable isotopes to measure energy expenditure in birds. After administration, the isotopes reach equilibrium within the body's bicarbonate pools at a fast rate due to the small size of the bicarbonate pool in relation to CO(2) flux. This technique is therefore ideal for measuring energy expenditure over short-term activities. The major advantage of this technique is that it can be applied without the animal having to wear a respirometry mask or being enclosed in a respirometry chamber. Despite the technique's suitability for use in birds and other animals, there have been few studies that have used it to date and so its potential is not fully understood. Here we discuss the methodology and review previous applications.
(13)C 标记的 NaHCO3 技术使用稳定同位素来测量鸟类的能量消耗。给药后，由于碳酸氢盐池相对于 CO2 通量较小，因此同位素会以很快的速度在体内的碳酸氢盐池中达到平衡。因此，该技术非常适合测量短期活动中的能量消耗。该技术的主要优点是，无需让动物佩戴呼吸代谢测量仪或置于呼吸代谢测量室中即可应用。尽管该技术适用于鸟类和其他动物，但迄今为止很少有研究使用该技术，因此其潜力尚未完全了解。在这里，我们讨论了该方法，并回顾了以前的应用。
