Department of Applied Chemistry, Faculty of Science, University of Mohaghegh Ardabili, University Street, Ardabil, Iran.
Anal Chim Acta. 2010 Jun 11;669(1-2):94-101. doi: 10.1016/j.aca.2010.04.044. Epub 2010 May 17.
A new molecularly imprinted polymer material having urea molecule selective cavities was introduced. Urea was properly dissolved in acetonitrile in the presence of an acidic functional monomer. Molecularly imprinted polymers with different compositions were examined and the roper formulation was selected. It was shown that the MIP had a considerable selectivity for urea in comparison to similar compounds such as thiourea and hydroxyurea. The obtained polymer was used as an adsorber for solid phase extraction (SPE) of urea in the aqueous samples. The extracted urea was determined by using a spectrophotometric method. Different parameters of SPE were optimized and the developed procedure was used for urea determination in real samples. The calibration graph of the method was linear in the range of 0.6-8.3 micromol L(-1). The detection limit was calculated to be 0.14 micromol L(-1).
引入了一种具有尿素分子选择腔的新型分子印迹聚合物材料。在酸性功能单体的存在下，将尿素适当地溶解在乙腈中。检查了具有不同组成的分子印迹聚合物，并选择了合适的配方。结果表明，与类似的化合物如硫脲和羟基脲相比，MIP 对尿素具有相当的选择性。所得聚合物被用作固相萃取（SPE）中用于在水溶液样品中提取尿素的吸附剂。通过分光光度法测定提取的尿素。优化了 SPE 的不同参数，并将开发的方法用于实际样品中尿素的测定。该方法的校准曲线在 0.6-8.3μmol L(-1)范围内呈线性。检测限计算为 0.14μmol L(-1)。
