对在南美洲分离出的腺病毒3型和7型的四种基因组类型进行限制酶切位点图谱分析。

Restriction site mapping of four genome types of adenovirus types 3 and 7 isolated in South America.

作者信息

Niel C, Moraes M T, Mistchenko A S, Leite J P, Gomes S A

机构信息

Institut National de la Santé et de la Recherche Médicale, U233 Virologie Moléculaire, Lille, France.

出版信息

J Med Virol. 1991 Feb;33(2):123-7. doi: 10.1002/jmv.1890330211.

DOI:10.1002/jmv.1890330211

PMID:2051141

Abstract

New genome types of Ad3 and Ad7 were found among adenovirus (Ad) strains isolated from stools of children during epidemiological surveys made in São Paulo, Brazil, and Buenos Aires, Argentina. These were characterized by DNA analysis with 11 restriction endonucleases and showed a number of new restriction patterns, notably for BamHI, BcII, BgIII, HindIII, KpnI, and SmaI. Restriction maps of the genome types, named Ad3e1, Ad3e2, Ad3h, and Ad7h, were constructed and compared with those of Ad3p and Ad7p.

摘要

在巴西圣保罗和阿根廷布宜诺斯艾利斯进行的流行病学调查中，从儿童粪便中分离出的腺病毒（Ad）菌株中发现了Ad3和Ad7的新基因组类型。通过用11种限制性内切酶进行DNA分析对这些新基因组类型进行了表征，并显示出许多新的限制性图谱，特别是针对BamHI、BcII、BgIII、HindIII、KpnI和SmaI的图谱。构建了名为Ad3e1、Ad3e2、Ad3h和Ad7h的基因组类型的限制性图谱，并与Ad3p和Ad7p的图谱进行了比较。