Golovina G I, Zolotaryov F N, Yurlova T I
All-Union Research Institute of Influenza, Leningrad, USSR.
J Clin Microbiol. 1991 Oct;29(10):2313-21. doi: 10.1128/jcm.29.10.2313-2321.1991.
An analysis of adenovirus strains isolated in the Soviet Union from 1976 to 1988 revealed four genome types of adenovirus type 3 (Ad3), i.e., Ad3a4, Ad3a9, Ad3a10, and Ad3a11, and four genome types of adenovirus type 7 (Ad7), i.e., Ad7p, Ad7a, Ad7a(1-5), and Ad7f1, identified with the DNA restriction enzymes BamHI, BglII, and HindIII. Three of them, Ad3a10, Ad3a11, and Ad7f1, are newly discovered. The genetic heterogeneity of adenoviruses was examined with restriction endonuclease Cfr13I with a 4-base recognition cleavage site. Eighteen different restriction patterns were identified among 21 selected Ad3 strains after cleavage of DNA with Cfr13I. Eight different subtypes were identified among 20 Ad7 strains by the same technique. For estimation of the relationships among these genome subtypes, pairwise analyses of comigrating DNA restriction fragments from isolates of Ad3 and Ad7 were done after digestion with Cfr13I or with restriction endonucleases recognizing DNA sequences of 6 bp. Surprisingly, the results were very discrepant.
对1976年至1988年在苏联分离出的腺病毒株进行分析后发现，3型腺病毒（Ad3）有四种基因组类型，即Ad3a4、Ad3a9、Ad3a10和Ad3a11，7型腺病毒（Ad7）有四种基因组类型，即Ad7p、Ad7a、Ad7a(1 - 5)和Ad7f1，这些是用DNA限制性内切酶BamHI、BglII和HindIII鉴定出来的。其中三种，即Ad3a10、Ad3a11和Ad7f1，是新发现的。用具有4碱基识别切割位点的限制性内切酶Cfr13I检测腺病毒的遗传异质性。在用Cfr13I切割DNA后，在21株选定的Ad3菌株中鉴定出18种不同的限制性图谱。通过相同技术在20株Ad7菌株中鉴定出8种不同的亚型。为了评估这些基因组亚型之间的关系，在用Cfr13I或识别6碱基DNA序列的限制性内切酶消化后，对Ad3和Ad7分离株中共同迁移的DNA限制性片段进行了成对分析。令人惊讶的是，结果差异很大。
