Adeyemi D O, Komolafe O A, Adewole O S, Obuotor E M, Abiodun A A, Adenowo T K
Department of Anatomy and Cell Biology, Obafemi Awolowo University, Il-Ife, Nigeria.
Folia Morphol (Warsz). 2010 May;69(2):92-100.
Microanatomical changes in the pancreatic islet cells of streptozotocin induced diabetic Wistar rats were studied after treatment with methanolic extracts of Annona muricata leaves. Thirty adult Wistar rats were randomly assigned into three groups (control, untreated diabetic group, and A. muricata-treated diabetic group) of ten rats each. Diabetes mellitus was experimentally induced in groups B and C by a single intra-peritoneal injection of 80 mg/kg streptozotocin dissolved in 0.1 M citrate buffer. The control rats were intraperitoneally injected with an equivalent volume of citrate buffer. Daily intra peritoneal injections of 100 mg/kg A. muricata were administered to group C rats for two weeks. Post sacrifice the pancreases of the rats were excised and fixed in Bouin's fluid. The tissues were processed for paraffin embedding and sections of 5 mum thickness were produced and stained with H & E, Gomori aldehyde fuchsin, and chrome alum haematoxylin-phloxine for demonstration of the beta-cells of islets of pancreatic islets. Histomorphological and morphometric examination of the stained pancreatic sections showed a significant increase in the number, diameter, and volume of the beta-cells of pancreatic islets of the A. muricata-treated group (5.67 +/- 0.184 N/1000 mum(2), 5.38 +/- 0.093 mum and 85.12 +/- 4.24 mum(3), respectively) when compared to that of the untreated diabetic group of rats (2.85 +/- 0.361 N/1000 mum(2), 2.85 +/- 0.362 mum and 69.56 +/- 5.216 mum(3), respectively). The results revealed regeneration of the beta-cells of islets of pancreatic islet of rats treated with extract of A. muricata.
研究了用番荔枝叶甲醇提取物处理后，链脲佐菌素诱导的糖尿病Wistar大鼠胰岛细胞的微观解剖学变化。30只成年Wistar大鼠被随机分为三组（对照组、未治疗的糖尿病组和番荔枝治疗的糖尿病组），每组10只。通过单次腹腔注射溶解于0.1M柠檬酸盐缓冲液中的80mg/kg链脲佐菌素，在B组和C组中实验性诱导糖尿病。对照组大鼠腹腔注射等量的柠檬酸盐缓冲液。每天给C组大鼠腹腔注射100mg/kg番荔枝，持续两周。处死后，切除大鼠的胰腺并固定在Bouin氏液中。对组织进行石蜡包埋，制作5μm厚的切片，并用苏木精和伊红、Gomori醛品红以及铬明矾苏木精-焰红染料染色，以显示胰岛的β细胞。对染色的胰腺切片进行组织形态学和形态计量学检查显示，与未治疗的糖尿病大鼠组相比，番荔枝治疗组大鼠胰岛β细胞的数量、直径和体积显著增加（分别为5.67±0.184个/1000μm²、5.38±0.093μm和85.12±4.24μm³）（未治疗的糖尿病大鼠组分别为2.85±0.361个/1000μm²、2.85±0.362μm和69.56±5.216μm³）。结果显示，用番荔枝提取物处理的大鼠胰岛β细胞发生了再生。
