Department of Neurosurgery, Methodist Hospital, Houston, TX 77030, USA.
Anal Biochem. 2010 Sep 15;404(2):118-26. doi: 10.1016/j.ab.2010.05.029. Epub 2010 Jun 1.
Fluorescence microscopy is a method widely used in life sciences to image biological processes in living and fixed cells or in fixed tissues. Quantification and calibration of images in fluorescence microscopy is notoriously difficult. We have developed a new methodology to prepare tissue "phantoms" that contain known amounts of (i) fluorophore, (ii) DNA, (iii) proteins, and (iv) DNA oligonucleotide standards. The basis of the phantoms is the ability of gelatin to act as a matrix for the conjugation of fluorophores as either a free-flowing liquid or a gelatinous solid depending on temperature (> or = 40 and < or = 4 degrees C).
荧光显微镜是一种在生命科学中广泛使用的方法，用于对活细胞和固定细胞或固定组织中的生物过程进行成像。荧光显微镜的图像定量和校准是众所周知的困难。我们开发了一种新的方法来制备组织“幻影”，其中包含已知量的（i）荧光染料，（ii）DNA，（iii）蛋白质和（iv）DNA 寡核苷酸标准。幻影的基础是明胶能够作为荧光染料的基质，根据温度（> = 40 和 < = 4 摄氏度），以自由流动的液体或凝胶状固体的形式存在。
