Sakeena M H F, Elrashid S M, Muthanna F A, Ghassan Z A, Kanakal M M, Laila Lia, Munavvar A S, Azmin M N
Department of Pharmaceutical Technology, School of Pharmaceutical Sciences, Universiti Sains, Malaysia.
J Oleo Sci. 2010;59(7):395-400. doi: 10.5650/jos.59.395.
This study sets out to investigate the in vitro permeation of ketoprofen from the formulated nanoemulsions through excised rat skin. In vitro permeation of ketoprofen nanoemulsion through rat skin was evaluated in Franz diffusion cells and compared with marketed product (Fastum gel). Limonene which has been reported to be a good enhancer for ketoprofen was selected. Moreover the effects of limonene which was added to the nanoemulsion formulations at levels of 1%, 2%, 3% and on rat skin permeation of ketoprofen were also evaluated. The selected optimized formulation was further studied for skin irritation. Utilization of limonene as a penetration enhancer increased the permeation of ketoprofen from the formulated nanoemulsion with increasing concentrations of limonene. The results obtained showed that nanoemulsion with 3% limonene produced similar and comparable skin permeation of ketoprofen with marketed formulation and the skin irritation study on rats showed the optimized formulation prepared was safe.
本研究旨在考察酮洛芬从所制备的纳米乳剂中通过离体大鼠皮肤的体外渗透情况。在Franz扩散池中评估了酮洛芬纳米乳剂通过大鼠皮肤的体外渗透，并与市售产品（法斯通凝胶）进行比较。选择了据报道对酮洛芬有良好增渗作用的柠檬烯。此外，还评估了在纳米乳剂配方中添加1%、2%、3%的柠檬烯对酮洛芬在大鼠皮肤渗透的影响。对所选的优化配方进一步进行皮肤刺激性研究。使用柠檬烯作为渗透促进剂，随着柠檬烯浓度的增加，酮洛芬从所制备的纳米乳剂中的渗透增加。所得结果表明，含3%柠檬烯的纳米乳剂产生的酮洛芬皮肤渗透与市售制剂相似且具有可比性，对大鼠的皮肤刺激性研究表明所制备的优化配方是安全的。
