University of Lausanne, Department of Fundamental Microbiology, 1015 Lausanne, Switzerland.
Nat Rev Microbiol. 2010 Jul;8(7):511-22. doi: 10.1038/nrmicro2392.
Bacteria have long been the targets for genetic manipulation, but more recently they have been synthetically designed to carry out specific tasks. Among the simplest of these tasks is chemical compound and toxicity detection coupled to the production of a quantifiable reporter signal. In this Review, we describe the current design of bacterial bioreporters and their use in a range of assays to measure the presence of harmful chemicals in water, air, soil, food or biological specimens. New trends for integrating synthetic biology and microengineering into the design of bacterial bioreporter platforms are also highlighted.
细菌一直是遗传操作的目标，但最近它们被人为设计用来执行特定的任务。在这些最简单的任务中，有化学化合物和毒性检测以及产生可量化的报告信号。在这篇综述中，我们描述了细菌生物传感器的当前设计及其在一系列测定水中、空气中、土壤中、食物中和生物样本中有害化学物质存在的应用。还强调了将合成生物学和微工程集成到细菌生物传感器平台设计中的新趋势。
