雌激素受体-α的一种变体，ER-α36 在人胃癌中表达，并与淋巴结转移高度相关。
A variant of estrogen receptor-alpha, ER-alpha36 is expressed in human gastric cancer and is highly correlated with lymph node metastasis.
机构信息
Department of Pathology and Pathophysiology, School of Medicine, Jianghan University, Wuhan, Hubei Province 430056, PR China.
出版信息
Oncol Rep. 2010 Jul;24(1):171-6.
Gastric cancer is one of the most common cancers in the world; almost two-thirds of gastric cancer cases and deaths occur in less developed regions. The molecular and cellular events during development of gastric cancer remain unclear. Herein, we examined the expression of ER-alpha36, an ER-alpha variant, in established gastric cancer cell lines and specimens from 22 gastric cancer patients. RT-PCR, Western blot and immunohistochemistry methods were used to assess expression levels of ER-alpha36. ER-alpha36 localization in gastric cancer cells was determined with an immunofluorenscence assay. Both mRNA and protein of ER-alpha36 were detected in all established gastric cancer cell lines examined. Higher levels of ER-alpha36 mRNA were expressed in 17 of 22 (77.27%) tumor specimens examined compared to the paired normal tissues (p<0.05). ER-alpha36 protein was expressed mainly on the plasma membrane and in the cytoplasm of the established gastric cancer cells. ER-alpha36 expression is highly correlated with lymph node metastasis in human gastric cancer (P<0.05). The estrogen receptor variant ER-alpha36 is highly expressed in human gastric cancer. ER-alpha36 expression may be used as a predictive marker for lymph node metastasis of gastric cancer.
胃癌是世界上最常见的癌症之一；几乎三分之二的胃癌病例和死亡发生在欠发达地区。胃癌发生过程中的分子和细胞事件仍不清楚。在此，我们研究了 ER-α36 在已建立的胃癌细胞系和 22 名胃癌患者标本中的表达。使用 RT-PCR、Western blot 和免疫组织化学方法评估 ER-α36 的表达水平。用免疫荧光测定法测定 ER-α36 在胃癌细胞中的定位。在检查的所有已建立的胃癌细胞系中均检测到 ER-α36 mRNA 和蛋白。与配对的正常组织相比，在检查的 22 个肿瘤标本中的 17 个（77.27%）中检测到 ER-α36 mRNA 水平更高（p<0.05）。ER-α36 蛋白主要表达在建立的胃癌细胞的质膜和细胞质上。ER-α36 表达与人胃癌的淋巴结转移高度相关（P<0.05）。雌激素受体变体 ER-α36 在人胃癌中高度表达。ER-α36 表达可作为预测胃癌淋巴结转移的标志物。
Zotero 插件