Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Council of Scientific and Industrial Research, Mysore, India.
Neurochem Res. 2010 Sep;35(9):1402-12. doi: 10.1007/s11064-010-0198-z. Epub 2010 Jun 1.
Creatine (Cr), an ergogenic nutritional supplement is demonstrated to possess bioenergetic, antiexcitotoxic and antioxidant properties. This study investigated the neuroprotective effects of Cr against rotenone induced oxidative stress, mortality and neurotoxicty in Drosophila melanogaster. We found significant diminution in the endogenous levels of oxidative markers in whole body homogenates of flies exposed to Cr (2-10 mM). Cr supplementation resulted in reduced mortality in flies exposed to rotenone (500 microM) and better performance in a negative geotaxis assay. Further Cr (10 mM) markedly offset rotenone induced mitochondrial oxidative stress, completely restored the GSH levels, nitric oxide levels, activity of Mn-SOD and dopamine depletion. In an oxidative stress bioassay, flies given Cr prophylaxis exhibited marked resistance to paraquat exposure. These data allow us to hypothesize that the neuroprotective action of Cr in Drosophila may be related to its direct antioxidant activity and ability to abrogate rotenone induced mitochondrial oxidative stress.
肌酸（Cr）是一种具有生物能量、抗兴奋毒性和抗氧化特性的运动营养补充剂。本研究探讨了 Cr 对黑腹果蝇中鱼藤酮诱导的氧化应激、死亡率和神经毒性的神经保护作用。我们发现，暴露于 Cr（2-10mM）的果蝇全身匀浆中的氧化标记物的内源性水平显著降低。Cr 补充剂可降低暴露于鱼藤酮（500μM）的果蝇的死亡率，并在负趋地性测定中表现出更好的性能。进一步的 Cr（10mM）显著减轻了鱼藤酮诱导的线粒体氧化应激，完全恢复了 GSH 水平、一氧化氮水平、Mn-SOD 活性和多巴胺耗竭。在氧化应激生物测定中，给予 Cr 预防的果蝇对百草枯暴露表现出明显的抗性。这些数据使我们假设 Cr 在果蝇中的神经保护作用可能与其直接的抗氧化活性和消除鱼藤酮诱导的线粒体氧化应激的能力有关。
