Aganovic-Musinovic Izeta, Prljaca-Zecevic Lamija, Subasic Djemo
Center for Genetics, Faculty of Medicine, University of Sarajevo, Cekalusa 80, 71000 Sarajevo, Bosnia and Herzegovina.
Med Arh. 2010;64(2):68-70.
While the SLE (Systemic Lupus Erythematosus) specificity of ANA is low, that of anti-dsDNA autoantibodies is high. The DNA used in the assay must be double stranded: autoantibodies to single-stranded (ss) DNA exist in many diseases and specific to none. The prevalence (70%) of anti-dsDNA autoantibodies is much higherin SLE, giving a higher diagnostic sensitivity than the similarly disease-specific anti-Sm autoantibodies (30%). Anti-dsDNA autoantibodies are usually detected by very analytically sensitive techniques, such as ELISA (Enzyme Linked Immunosorbent Assay). Within SLE, ds-DNA autoantibodies tend to associate with the presence of glomerulonephritis. Their levels are used to monitor disease activity. We suggest the use of ds-DNA to find the difference between SLE patientswith benign variants and classical syndrome of severe skin and renal disease.
虽然抗核抗体（ANA）对系统性红斑狼疮（SLE）的特异性较低，但抗双链DNA自身抗体的特异性较高。检测中使用的DNA必须是双链的：单链（ss）DNA自身抗体存在于多种疾病中，不具有疾病特异性。抗双链DNA自身抗体在SLE中的患病率（70%）要高得多，比同样具有疾病特异性的抗Sm自身抗体（30%）具有更高的诊断敏感性。抗双链DNA自身抗体通常通过分析灵敏度很高的技术检测，如酶联免疫吸附测定（ELISA）。在SLE患者中，双链DNA自身抗体往往与肾小球肾炎的存在相关。其水平用于监测疾病活动。我们建议使用双链DNA来区分患有良性变异的SLE患者和患有严重皮肤及肾脏疾病的经典综合征患者。
