Clarke M C, Carr R, Burdash N M, Chen Z Y, Ainsworth S K
Diagn Immunol. 1986;4(6):288-93.
Systemic lupus erythematosus (SLE) is a multisystem disorder accompanied by a diverse spectrum of serum autoantibodies. Antibodies to double stranded DNA (dsDNA) are considered to be the most specific marker for this disease. In this study the results obtained from three different assays for dsDNA are compared: an indirect fluorescence antibody assay (IFA), a radioimmunoassay (RIA) and, an enzyme-linked immunosorbent assay (ELISA), on 57 SLE sera and 28 Sera from other disorders. Correlation of these anti-DNA results are made with C3, C4, and antinuclear antibody (ANA) titers. Our results show the IFA assay is the most sensitive and the least specific of the three tests. The RIA was found to be the most specific and was approximately as sensitive as the ELISA. We also found significant inverse correlations between anti-dsDNA levels and circulating complement levels among SLE sera for all three assays. ANA titers were significantly correlated with all anti-dsDNA assays as well. However, these anti-dsDNA assays show only modest differences explainable by numerous mechanisms. Hence, a clearly superior anti-dsDNA method does not emerge from our study.
系统性红斑狼疮（SLE）是一种多系统疾病，伴有多种血清自身抗体。双链DNA（dsDNA）抗体被认为是该疾病最具特异性的标志物。在本研究中，比较了对57份SLE血清和28份其他疾病血清进行的三种不同dsDNA检测方法的结果：间接荧光抗体法（IFA）、放射免疫法（RIA）和酶联免疫吸附测定法（ELISA）。将这些抗DNA结果与C3、C4和抗核抗体（ANA）滴度进行相关性分析。我们的结果表明，IFA检测是三种检测中最敏感但特异性最低的。发现RIA是最具特异性的，其敏感性与ELISA大致相同。我们还发现，在所有三种检测中，SLE血清中抗dsDNA水平与循环补体水平之间存在显著的负相关。ANA滴度与所有抗dsDNA检测也显著相关。然而，这些抗dsDNA检测仅显示出由多种机制导致的适度差异。因此，我们的研究并未得出明显更优的抗dsDNA方法。
