复制蛋白 A：在复制和修复的交叉路口指挥交通。
Replication protein A: directing traffic at the intersection of replication and repair.
机构信息
College of Dentistry, University of Nebraska Medical Center, Lincoln, Nebraska 68583, USA.
出版信息
Front Biosci (Landmark Ed). 2010 Jun 1;15(3):883-900. doi: 10.2741/3652.
Abstract
Since the initial discovery of replication protein A (RPA) as a DNA replication factor, much progress has been made on elucidating critical roles for RPA in other DNA metabolic pathways. RPA has been shown to be required for DNA replication, DNA repair, DNA recombination, and the DNA damage response pathway with roles in checkpoint activation. This review summarizes the current understanding of RPA structure, phosphorylation and protein-protein interactions in mediating these DNA metabolic processes.
摘要
自最初发现复制蛋白 A (RPA) 作为一种 DNA 复制因子以来，在阐明 RPA 在其他 DNA 代谢途径中的关键作用方面已经取得了很大进展。已经表明 RPA 对于 DNA 复制、DNA 修复、DNA 重组以及在检查点激活中发挥作用的 DNA 损伤反应途径是必需的。这篇综述总结了目前对 RPA 结构、磷酸化和蛋白质-蛋白质相互作用在介导这些 DNA 代谢过程中的理解。
相似文献
1
Replication protein A: directing traffic at the intersection of replication and repair.复制蛋白 A：在复制和修复的交叉路口指挥交通。
Front Biosci (Landmark Ed). 2010 Jun 1;15(3):883-900. doi: 10.2741/3652.
2
[Replication protein A as a major eukaryotic single-stranded DNA-binding protein and its role in DNA repair].[复制蛋白A作为主要的真核单链DNA结合蛋白及其在DNA修复中的作用]
Mol Biol (Mosk). 2016 Sep-Oct;50(5):735-750. doi: 10.7868/S0026898416030083.
3
Replication protein A: single-stranded DNA's first responder: dynamic DNA-interactions allow replication protein A to direct single-strand DNA intermediates into different pathways for synthesis or repair.复制蛋白A：单链DNA的首要应答者：动态的DNA相互作用使复制蛋白A能够将单链DNA中间体导向不同的合成或修复途径。
Bioessays. 2014 Dec;36(12):1156-61. doi: 10.1002/bies.201400107. Epub 2014 Aug 29.
4
Interactive Roles of DNA Helicases and Translocases with the Single-Stranded DNA Binding Protein RPA in Nucleic Acid Metabolism.DNA解旋酶和转位酶与单链DNA结合蛋白RPA在核酸代谢中的相互作用角色
Int J Mol Sci. 2017 Jun 8;18(6):1233. doi: 10.3390/ijms18061233.
5
Functions of human replication protein A (RPA): from DNA replication to DNA damage and stress responses.人类复制蛋白A（RPA）的功能：从DNA复制到DNA损伤及应激反应
J Cell Physiol. 2006 Aug;208(2):267-73. doi: 10.1002/jcp.20622.
6
Dynamic elements of replication protein A at the crossroads of DNA replication, recombination, and repair.复制蛋白 A 的动态元件在 DNA 复制、重组和修复的交汇点。
Crit Rev Biochem Mol Biol. 2020 Oct;55(5):482-507. doi: 10.1080/10409238.2020.1813070. Epub 2020 Aug 28.
7
Replication-mediated disassociation of replication protein A-XPA complex upon DNA damage: implications for RPA handing off.复制介导的复制蛋白 A-XPA 复合物在 DNA 损伤下的解离：对 RPA 交接的影响。
Cell Biol Int. 2012 Aug 1;36(8):713-20. doi: 10.1042/CBI20110633.
8
Single-stranded DNA damage: Protecting the single-stranded DNA from chemical attack.单链 DNA 损伤：保护单链 DNA 免受化学攻击。
DNA Repair (Amst). 2020 Mar;87:102804. doi: 10.1016/j.dnarep.2020.102804. Epub 2020 Jan 20.
9
Photoreactive DNA as a Tool to Study Replication Protein A Functioning in DNA Replication and Repair.光反应性 DNA 作为研究复制蛋白在 DNA 复制和修复中功能的工具。
Photochem Photobiol. 2020 Mar;96(2):440-449. doi: 10.1111/php.13222. Epub 2020 Mar 5.
10
Repair-specific functions of replication protein A.复制蛋白 A 的修复特异性功能。
J Biol Chem. 2012 Feb 3;287(6):3908-18. doi: 10.1074/jbc.M111.287441. Epub 2011 Dec 16.
引用本文的文献
1
The human primosome requires replication protein A when copying DNA with inverted repeats.人类引发体在复制具有反向重复序列的DNA时需要复制蛋白A。
Nucleic Acids Res. 2025 Aug 11;53(15). doi: 10.1093/nar/gkaf799.
2
Biochemical impact of p300-mediated acetylation of replication protein A: Implications for DNA metabolic pathway choice.p300介导的复制蛋白A乙酰化的生化影响：对DNA代谢途径选择的意义
J Biol Chem. 2025 May 17;301(6):110250. doi: 10.1016/j.jbc.2025.110250.
3
Primordial germ cell DNA demethylation and development require DNA translesion synthesis.原始生殖细胞DNA去甲基化与发育需要DNA跨损伤合成。
Nat Commun. 2024 May 3;15(1):3734. doi: 10.1038/s41467-024-47219-2.
4
The Intriguing Mystery of RPA Phosphorylation in DNA Double-Strand Break Repair.RPA 磷酸化在 DNA 双链断裂修复中的迷人之谜。
Genes (Basel). 2024 Jan 27;15(2):167. doi: 10.3390/genes15020167.
5
Role of RPA Phosphorylation in the ATR-Dependent G2 Cell Cycle Checkpoint.RPA 磷酸化在 ATR 依赖性 G2 细胞周期检查点中的作用。
Genes (Basel). 2023 Dec 13;14(12):2205. doi: 10.3390/genes14122205.
6
HELQ as a DNA helicase: Its novel role in normal cell function and tumorigenesis (Review).HELQ 作为一种 DNA 解旋酶：其在正常细胞功能和肿瘤发生中的新作用（综述）。
Oncol Rep. 2023 Dec;50(6). doi: 10.3892/or.2023.8657. Epub 2023 Nov 3.
7
Antagonistic roles of canonical and Alternative-RPA in disease-associated tandem CAG repeat instability.经典 RPA 和 Alternative-RPA 在与疾病相关的串联 CAG 重复不稳定中的拮抗作用。
Cell. 2023 Oct 26;186(22):4898-4919.e25. doi: 10.1016/j.cell.2023.09.008. Epub 2023 Oct 11.
8
RPA-like single-stranded DNA-binding protein complexes including CST serve as specialized processivity factors for polymerases.RPA 样单链 DNA 结合蛋白复合物包括 CST 作为聚合酶的专门延伸因子。
Curr Opin Struct Biol. 2023 Aug;81:102611. doi: 10.1016/j.sbi.2023.102611. Epub 2023 May 26.
9
Signalling inhibition by ponatinib disrupts productive alternative lengthening of telomeres (ALT).帕纳替尼通过信号抑制破坏端粒的有效替代性延长（ALT）。
Nat Commun. 2023 Apr 6;14(1):1919. doi: 10.1038/s41467-023-37633-3.
10
A DNA damage-induced phosphorylation circuit enhances Mec1 Ddc2 recruitment to Replication Protein A.一个 DNA 损伤诱导的磷酸化回路增强了 Mec1 Ddc2 向复制蛋白 A 的募集。
Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2300150120. doi: 10.1073/pnas.2300150120. Epub 2023 Mar 30.
本文引用的文献
1
SSB protein diffusion on single-stranded DNA stimulates RecA filament formation.单链DNA上的SSB蛋白扩散刺激RecA丝状体形成。
Nature. 2009 Oct 22;461(7267):1092-7. doi: 10.1038/nature08442. Epub 2009 Oct 11.
2
A supramodular FHA/BRCT-repeat architecture mediates Nbs1 adaptor function in response to DNA damage.一种超模块 FHA/BRCT 重复结构介导 Nbs1 衔接蛋白在 DNA 损伤应答中的功能。
Cell. 2009 Oct 2;139(1):100-11. doi: 10.1016/j.cell.2009.07.043.
3
Nbs1 flexibly tethers Ctp1 and Mre11-Rad50 to coordinate DNA double-strand break processing and repair.Nbs1灵活地连接Ctp1和Mre11-Rad50，以协调DNA双链断裂的处理和修复。
Cell. 2009 Oct 2;139(1):87-99. doi: 10.1016/j.cell.2009.07.033.
4
The annealing helicase HARP protects stalled replication forks.退火解旋酶HARP可保护停滞的复制叉。
Genes Dev. 2009 Oct 15;23(20):2394-9. doi: 10.1101/gad.1836409. Epub 2009 Sep 30.
5
The annealing helicase HARP is recruited to DNA repair sites via an interaction with RPA.退火解旋酶HARP通过与RPA的相互作用被招募到DNA修复位点。
Genes Dev. 2009 Oct 15;23(20):2400-4. doi: 10.1101/gad.1831509. Epub 2009 Sep 30.
6
The SIOD disorder protein SMARCAL1 is an RPA-interacting protein involved in replication fork restart.SIOD 疾病蛋白 SMARCAL1 是一种与复制蛋白 A（RPA）相互作用的蛋白，参与复制叉重启。
Genes Dev. 2009 Oct 15;23(20):2415-25. doi: 10.1101/gad.1832309. Epub 2009 Sep 30.
7
The annealing helicase SMARCAL1 maintains genome integrity at stalled replication forks.退火解旋酶SMARCAL1在停滞的复制叉处维持基因组完整性。
Genes Dev. 2009 Oct 15;23(20):2405-14. doi: 10.1101/gad.1839909. Epub 2009 Sep 30.
8
Hyperphosphorylation of replication protein A in cisplatin-resistant and -sensitive head and neck squamous cell carcinoma cell lines.顺铂耐药和敏感的头颈部鳞状细胞癌细胞系中复制蛋白 A 的过度磷酸化。
Head Neck. 2010 May;32(5):636-45. doi: 10.1002/hed.21234.
9
Ionizing radiation-dependent and independent phosphorylation of the 32-kDa subunit of replication protein A during mitosis.有丝分裂期间复制蛋白A 32-kDa亚基的电离辐射依赖性和非依赖性磷酸化
Nucleic Acids Res. 2009 Oct;37(18):6028-41. doi: 10.1093/nar/gkp605. Epub 2009 Aug 11.
10
Prediction of and experimental support for the three-dimensional structure of replication protein A.复制蛋白A三维结构的预测及实验支持
Biochemistry. 2009 Aug 25;48(33):7892-905. doi: 10.1021/bi801896s.
Zotero 插件