Department of Biochemistry, University of Colorado Bouder, Boulder, CO 80309, USA.
Department of Biochemistry, University of Colorado Bouder, Boulder, CO 80309, USA.
Curr Opin Struct Biol. 2023 Aug;81:102611. doi: 10.1016/j.sbi.2023.102611. Epub 2023 May 26.
Telomeres and other single-stranded regions of the genome require specialized management to maintain stability and for proper progression of DNA metabolism pathways. Human Replication Protein A and CTC1-STN1-TEN1 are structurally similar heterotrimeric protein complexes that have essential ssDNA-binding roles in DNA replication, repair, and telomeres. Yeast and ciliates have related ssDNA-binding proteins with strikingly conserved structural features to these human heterotrimeric protein complexes. Recent breakthrough structures have extended our understanding of these commonalities by illuminating a common mechanism used by these proteins to act as processivity factors for their partner polymerases through their ability to manage ssDNA.
端粒和基因组的其他单链区域需要专门的管理来维持稳定性,并使 DNA 代谢途径正常进行。人类复制蛋白 A 和 CTC1-STN1-TEN1 是结构相似的异源三聚体蛋白复合物,在 DNA 复制、修复和端粒中具有必需的 ssDNA 结合作用。酵母和纤毛类动物具有与其同源的 ssDNA 结合蛋白,这些蛋白具有与人类异源三聚体蛋白复合物显著保守的结构特征。最近的突破性结构揭示了这些蛋白作为其聚合酶的持续因子的共同机制,从而扩展了我们对这些共性的理解,通过它们管理 ssDNA 的能力。