[用放射免疫分析法测定抗微粒体抗体和抗甲状腺球蛋白抗体]

[Measurement of anti-microsomal and anti-thyroglobulin antibodies by radioimmunoassay].

作者信息

Nakamura H, Mikami Y, Aono Y, Yoshinoya S, Uchimura H, Okubo A, Kitamura M, Kubota K, Sasaki N, Takaku F

机构信息

Central Clinical Laboratories, Tokyo University School of Medicine.

出版信息

Rinsho Byori. 1991 Apr;39(4):373-8.

PMID:2051616

Abstract

In this study, we measured anti-microsomal (same as anti-thyroid peroxidase) and anti-thyroglobulin antibodies (anti-TPOAb and anti-TgAb) in sera by new RIA kits and compared with the conventional particle agglutination (PA) kits. Two of 80 normals (2.5%) showed anti-TPOAb positive activity (greater than or equal to 0.3 unit/ml), and 9 of same samples (11.3%) showed anti TgAb positive activity (greater than or equal to 0.3 unit/ml). Sixty of 67 patients with Graves' disease (89.6%), and 14 of 22 with Hashimoto's disease (63.6%) were anti-TPOAb positive, and 42 (62.7%), and 18 (81.8%) were anti-TgAb positive respectively. Samples of 5 non-autoimmune disease were all negative by TPOAb and TgAb assays, suggesting the high specificity of new RIA kits to the autoimmune thyroid diseases. The RIA and PA methods showed a good correlation (anti-TPOAb:rs = 0.9134, anti-TgAb:rs = 0.7016). However, several negative sera in PA assay showed positive values in the RIA, so it was showed that new RIA kits were more sensitive than the PA assays.

摘要

在本研究中，我们使用新型放射免疫分析（RIA）试剂盒检测血清中的抗微粒体抗体（与抗甲状腺过氧化物酶相同）和抗甲状腺球蛋白抗体（抗TPOAb和抗TgAb），并与传统的颗粒凝集（PA）试剂盒进行比较。80名正常人中有2名（2.5%）抗TPOAb呈阳性（大于或等于0.3单位/毫升），同一样本中有9名（11.3%）抗TgAb呈阳性（大于或等于0.3单位/毫升）。67例格雷夫斯病患者中有60例（89.6%），22例桥本氏病患者中有14例（63.6%）抗TPOAb呈阳性，分别有42例（62.7%）和18例（81.8%）抗TgAb呈阳性。5例非自身免疫性疾病样本的TPOAb和TgAb检测均为阴性，表明新型RIA试剂盒对自身免疫性甲状腺疾病具有高特异性。RIA法和PA法显示出良好的相关性（抗TPOAb：rs = 0.9134，抗TgAb：rs = 0.7016）。然而，PA检测中的几份阴性血清在RIA中显示为阳性值，因此表明新型RIA试剂盒比PA检测更敏感。