Hyodoh F, Kinugawa K, Ueki A
Department of Hygiene, Kawasaki Medical School, Kurashiki.
Nihon Eiseigaku Zasshi. 1991 Feb;45(6):1074-81. doi: 10.1265/jjh.45.1074.
It is well known that persons exposed to asbestos display systemic immunological alterations: impaired lymphocyte response to PHA, the appearance of autoantibodies and elevation of the serum immunoglobulin level. The authors intend in this report to determine whether asbestos fibres (crocidolite, chrysotile and amosite) have any effect on the cell cycle of human lymphocytes after PHA stimulation. Peripheral blood mononuclear (PBM) cells were incubated with 10 micrograms/ml PHA for 2 days. After PHA stimulation, a decrease in the percentage of cells in the G0 phase and an increase in that of cells in the G1A, G1B and S phases was observed. When asbestos fibres or titanium dioxide (TiO2) was added to the culture dish at the beginning of the experiment, the progression of the cell cycle was inhibited only by asbestos fibres, in which case the percentage of cells in the G0 phase was significantly increased, while those of cells in the G1B and S phases were significantly decreased. The experiment for determining the critical period for inhibition of blastogenic response revealed that no inhibition was demonstrable when crocidolite fibre was added at 24 hr after PHA stimulation. Although the mechanisms of asbestos-fibre-mediated suppression remain to be clarified, these results showed that asbestos fibres act at an early stage (G0 phase) of the cell cycle and suppress the PHA stimulation of PBM cells.
众所周知，接触石棉的人会出现全身性免疫改变：淋巴细胞对PHA的反应受损、自身抗体出现以及血清免疫球蛋白水平升高。在本报告中，作者旨在确定石棉纤维（青石棉、温石棉和铁石棉）在PHA刺激后是否对人淋巴细胞的细胞周期有任何影响。将外周血单核（PBM）细胞与10微克/毫升PHA孵育2天。PHA刺激后，观察到G0期细胞百分比下降，G1A、G1B和S期细胞百分比增加。当在实验开始时将石棉纤维或二氧化钛（TiO2）添加到培养皿中时，细胞周期进程仅受到石棉纤维的抑制，在这种情况下，G0期细胞百分比显著增加，而G1B和S期细胞百分比显著下降。确定抑制增殖反应关键期的实验表明，在PHA刺激后24小时添加青石棉纤维时未显示出抑制作用。尽管石棉纤维介导的抑制机制仍有待阐明，但这些结果表明，石棉纤维作用于细胞周期的早期（G0期），并抑制PBM细胞的PHA刺激。
