大肠杆菌葡萄糖转运蛋白EIICGlc结构域的表达、纯化、结晶及初步X射线分析
Expression, purification, crystallization and preliminary X-ray analysis of the EIICGlc domain of the Escherichia coli glucose transporter.
作者信息
Zurbriggen Andreas, Schneider Philipp, Bähler Priska, Baumann Ulrich, Erni Bernhard
机构信息
Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jun 1;66(Pt 6):684-8. doi: 10.1107/S1744309110013102. Epub 2010 May 26.
Abstract
The glucose-import system of Escherichia coli consists of a hydrophilic EIIA(Glc) subunit and a transmembrane EIICB(Glc) subunit. EIICB(Glc) (UniProt P69786) contains two domains: the transmembrane EIIC(Glc) domain (40.6 kDa) and the cytoplasmic EIIB(Glc) domain (8.0 kDa), which are fused by a linker that is strongly conserved among its orthologues. The EIICB(Glc) subunit can be split within this motif by trypsin. Here, the crystallization of the tryptic EIIC(Glc) domain is described. A complete data set was collected to 4.5 A resolution at 100 K.
摘要
大肠杆菌的葡萄糖导入系统由一个亲水性的EIIA(Glc)亚基和一个跨膜的EIICB(Glc)亚基组成。EIICB(Glc)（通用蛋白质数据库编号P69786）包含两个结构域：跨膜的EIIC(Glc)结构域（40.6 kDa）和细胞质中的EIIB(Glc)结构域（8.0 kDa），它们由一个在其直系同源物中高度保守的连接子融合在一起。EIICB(Glc)亚基可被胰蛋白酶在该基序内切割。在此，描述了胰蛋白酶切割后的EIIC(Glc)结构域的结晶过程。在100 K下收集了完整的数据集，分辨率达到4.5 Å。
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