氨基酸和甘氨酸乙酯作为溶菌酶的新型结晶试剂。
Amino acids and glycine ethyl ester as new crystallization reagents for lysozyme.
作者信息
Ito Len, Shiraki Kentaro, Yamaguchi Hiroshi
机构信息
Japan Synchrotron Radiation Research Institute (SPring-8), Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jun 1;66(Pt 6):750-4. doi: 10.1107/S174430911001376X. Epub 2010 May 27.
Abstract
Several amino acids and their derivatives are prominent additives in the field of protein chemistry. This study reports the use of charged amino acids and glycine ethyl ester as precipitants in protein crystallization, using hen egg-white lysozyme (HEWL) as a model. A discussion of the crystallization of HEWL using these reagents as precipitating agents is given.
摘要
几种氨基酸及其衍生物是蛋白质化学领域中重要的添加剂。本研究报道了使用带电荷的氨基酸和甘氨酸乙酯作为蛋白质结晶中的沉淀剂，以鸡蛋清溶菌酶（HEWL）作为模型。给出了使用这些试剂作为沉淀剂时HEWL的结晶情况的讨论。
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