Domingo J L, Gomez M, Llobet J M, Corbella J
Laboratory of Toxicology and Biochemistry, School of Medicine, University of Barcelona, Reus, Spain.
Kidney Int. 1991 Apr;39(4):598-601. doi: 10.1038/ki.1991.70.
Eight groups of female Sprague-Dawley rats were treated with 281 mg Al(OH)3/kg/day by gastric intubation five times a week for five weeks. Concurrently, animals in seven groups received ascorbic acid (56.3 mg/kg/day), citric acid (62 mg/kg/day), gluconic acid (62.7 mg/kg/day), lactic acid (28.8 mg/kg/day), malic acid (42.9 mg/kg/day), oxalic acid (28.8 mg/kg/day), and tartaric acid (48 mg/kg/day) in the drinking water. The eighth group did not receive any dietary constituent in the water and was designated as the control group. Animals were placed in plastic metabolic cages and urine was collected during the treatment period. The liver, spleen, kidney, brain and bone aluminum levels of each rat were measured, as well as the total amount of aluminum excreted into urine. All the dietary constituents significantly increased the aluminum concentrations in most of the tissues, with ascorbic and citric acids showing the highest rate of aluminum accumulation. In contrast, no significant differences between control and treated rats were observed in the concentrations of aluminum excreted into urine. In view of these results, we suggest that the effects of the simultaneous ingestion of aluminum hydroxide and those dietary constituents in uremic animals should be evaluated. Meanwhile, the diet of uremic patients should be carefully monitored.
将八组雌性斯普拉格-道利大鼠每周经胃插管给予281毫克氢氧化铝/千克/天，共五次，持续五周。同时，七组动物在饮用水中分别摄入抗坏血酸（56.3毫克/千克/天）、柠檬酸（62毫克/千克/天）、葡萄糖酸（62.7毫克/千克/天）、乳酸（28.8毫克/千克/天）、苹果酸（42.9毫克/千克/天）、草酸（28.8毫克/千克/天）和酒石酸（48毫克/千克/天）。第八组在水中不摄入任何膳食成分，被指定为对照组。将动物置于塑料代谢笼中，在治疗期间收集尿液。测量每只大鼠肝脏、脾脏、肾脏、大脑和骨骼中的铝含量，以及排泄到尿液中的铝总量。所有膳食成分均显著增加了大多数组织中的铝浓度，抗坏血酸和柠檬酸的铝积累率最高。相比之下，对照组和处理组大鼠排泄到尿液中的铝浓度没有显著差异。鉴于这些结果，我们建议应评估尿毒症动物同时摄入氢氧化铝和这些膳食成分的影响。同时，应对尿毒症患者的饮食进行仔细监测。
