Lai Jian-Cheng, Zhang Ying-Ying, Li Zhen-Hua, Jiang Hai-Jiao, He An-Zhi
School of Science, Nanjing University of Science & Technology, Nanjing 210094, China.
Appl Opt. 2010 Jun 1;49(16):3235-8. doi: 10.1364/AO.49.003235.
To apply reflection ellipsometry to determine the real and imaginary parts of the refractive index of biological tissues simultaneously, we combine reflection ellipsometry with total internal reflection to warrant minimal influences by the strong scattering and absorption of biological tissues. A K9 glass prism with refractive index 1.51468 at wavelength 632.8 nm and a Glan prism polarizer with an angular sampling interval of 0.1 degrees were used in our experimental setup. Using the setup, the complex refractive indices of some typical mammalian tissues were measured under the wavelength of 632.8 nm. The results show that the indices of porcine muscle, liver, pancreas, and dermis tissues were 1.3713+0.062i, 1.3791+0.0087i, 1.3517+0.0113i, and 1.3818+0.0049i, respectively.
为了应用反射椭圆偏振法同时测定生物组织折射率的实部和虚部，我们将反射椭圆偏振法与全内反射相结合，以确保生物组织的强散射和吸收产生的影响最小。我们的实验装置中使用了在波长632.8 nm处折射率为1.51468的K9玻璃棱镜和角度采样间隔为0.1度的格兰棱镜偏振器。利用该装置，在632.8 nm波长下测量了一些典型哺乳动物组织的复折射率。结果表明，猪肌肉、肝脏、胰腺和真皮组织的折射率分别为1.3713+0.062i、1.3791+0.0087i、1.3517+0.0113i和1.3818+0.0049i。
