测量整个血液及其成分在连续光谱区域的折射率。

Nankai University, School of Physics and TEDA Applied Physics, Key Laboratory of Weak-Light Nonlinea, China.

National Institute of Metrology, Division of Optics, Beijing, China.

J Biomed Opt. 2019 Mar;24(3):1-5. doi: 10.1117/1.JBO.24.3.035003.

The refractive index of blood is a key biophysical parameter, which can reflect the physiological state. We measured the refractive index of whole blood and other components, such as serum, plasma, and hemoglobin, based on internal reflection by using a homemade apparatus in the spectral range of 400 to 750 nm. In addition to the hemoglobin solution, which has a Soret band about 420 nm and two Q-bands between 500 and 600 nm, the measurements of other samples are the normal dispersion curve. The results are approximated by the Cauchy equation and Sellmeier equation, and the correlation coefficients are more than 0.997.

血液的折射率是一个关键的生物物理参数，可以反映生理状态。我们使用自制的仪器在 400 到 750nm 的光谱范围内基于内部反射测量了全血以及其他成分（如血清、血浆和血红蛋白）的折射率。除了血红蛋白溶液在 420nm 左右有一个 Soret 带和在 500 到 600nm 之间有两个 Q 带之外，其他样品的测量结果都是正常的色散曲线。结果用 Cauchy 方程和Sellmeier 方程近似，相关系数都大于 0.997。