Hare W R, Wahle K W
Rowett Research Institute, Bucksburn, Aberdeen, United Kingdom.
Lipids. 1991 Feb;26(2):102-6. doi: 10.1007/BF02544002.
The removal of soluble components from an ovine hepatic microsomal preparation decreased the omega-hydroxylation of dodecanoic and hexadecanoic acids. The results suggest that one or more soluble components play a role in the microsomal omega-hydroxylation of fatty acids. The possible roles in the reaction of catalase (known to stimulate the microsomal desaturations of fatty acids and alkylglycerols) and superoxide dismutase were investigated. The addition of these enzymes to the complete (but not the washed) microsomal preparation stimulated both the initial omega-hydroxylation reaction and the subsequent dehydrogenation reactions of the omega-oxidation pathway. The similarity of the effects of catalase and superoxide dismutase and stimulation of two different steps of the omega-oxidation pathway suggest that these agents are acting indirectly by removing active oxygen species rather than directly on the enzymes of microsomal fatty acid omega-hydroxylation.
从绵羊肝微粒体制剂中去除可溶性成分会降低十二烷酸和十六烷酸的ω-羟基化作用。结果表明，一种或多种可溶性成分在脂肪酸的微粒体ω-羟基化过程中发挥作用。研究了过氧化氢酶（已知可刺激脂肪酸和烷基甘油的微粒体去饱和作用）和超氧化物歧化酶在该反应中的可能作用。将这些酶添加到完整的（而非洗涤过的）微粒体制剂中，会刺激ω-羟基化初始反应以及ω-氧化途径随后的脱氢反应。过氧化氢酶和超氧化物歧化酶作用的相似性以及对ω-氧化途径两个不同步骤的刺激表明，这些试剂是通过去除活性氧间接起作用，而非直接作用于微粒体脂肪酸ω-羟基化的酶。
