Nouchi T, Tanaka Y, Tsukada T, Sato C, Marumo F
Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Liver. 1991 Apr;11(2):100-5. doi: 10.1111/j.1600-0676.1991.tb00499.x.
The appearance of alpha-smooth-muscle-actin (alpha-smA)-positive cells during hepatic fibrosis was studied immunohistochemically in rat and human livers. In the normal rat liver, alpha-smA was observed only in vascular smooth muscle cells. With the progression of fibrosis induced by CCl4 injection, alpha-smA-positive cells appeared in the perisinusoidal space and the fibrous septa, and ultimately surrounded regenerative nodules. An increase of desmin-positive cells was recognized in the fibrotic areas and the perisinusoidal area. In the human liver, alpha-smA-positive cells appeared in the fibrotic area, whereas no desmin-positive cells were observed, except in vascular walls of the central vein and the portal tract, alpha-smA is a good marker for the detection of myofibroblast-like cells, and the appearance of alpha-smA in liver mesenchymal cells seems closely related to the process of hepatic fibrosis in both rat and man.
采用免疫组织化学方法，在大鼠和人类肝脏中研究了肝纤维化过程中α-平滑肌肌动蛋白（α-smA）阳性细胞的出现情况。在正常大鼠肝脏中，α-smA仅在血管平滑肌细胞中观察到。随着四氯化碳注射诱导的纤维化进展，α-smA阳性细胞出现在窦周间隙和纤维间隔中，并最终包围再生结节。在纤维化区域和窦周区域可观察到结蛋白阳性细胞增加。在人类肝脏中，α-smA阳性细胞出现在纤维化区域，而除中央静脉和门静脉的血管壁外，未观察到结蛋白阳性细胞。α-smA是检测肌成纤维细胞样细胞的良好标志物，肝脏间充质细胞中α-smA的出现似乎与大鼠和人类的肝纤维化过程密切相关。
