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纤维中特异表达并参与细胞伸长的六个棉花 14-3-3 互作组分析。

Interactome analysis of the six cotton 14-3-3s that are preferentially expressed in fibres and involved in cell elongation.

机构信息

Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, HuaZhong Normal University, Wuhan 430079, China.

出版信息

J Exp Bot. 2010 Jul;61(12):3331-44. doi: 10.1093/jxb/erq155. Epub 2010 Jun 2.

DOI:10.1093/jxb/erq155
PMID:20519337
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2905198/
Abstract

Proteins of the 14-3-3 family regulate a divergent set of signalling pathways in all eukaryotic organisms. In this study, several cDNAs encoding 14-3-3 proteins were isolated from a cotton fibre cDNA library. The Gh14-3-3 genes share high sequence homology at the nucleotide level in the coding region and at the amino acid level. Real-time quantitative RT-PCR analysis indicated that the expression of these Gh14-3-3 genes is developmentally regulated in fibres, and reached their peak at the stage of rapid cell elongation of fibre development. Furthermore, overexpression of Gh14-3-3a, Gh14-3-3e, and Gh14-3-3L in fission yeast promoted atypical longitudinal growth of the host cells. Yeast two-hybrid analysis revealed that the interaction between cotton 14-3-3 proteins is isoform selective. Through yeast two-hybrid screening, 38 novel interaction partners of the six 14-3-3 proteins (Gh14-3-3a, Gh14-3-3e, Gh14-3-3f, Gh14-3-3g, Gh14-3-3h, and Gh14-3-3L), which are involved in plant development, metabolism, signalling transduction, and other cellular processes, were identified in cotton fibres. Taking these data together, it is proposed that the Gh14-3-3 proteins may participate in regulation of fibre cell elongation. Thus, the results of this study provide novel insights into the 14-3-3 signalling related to fibre development of cotton.

摘要

14-3-3 家族蛋白在所有真核生物中调节一组不同的信号通路。在这项研究中，从棉花纤维 cDNA 文库中分离出几个编码 14-3-3 蛋白的 cDNA。Gh14-3-3 基因在编码区的核苷酸水平和氨基酸水平上具有高度的序列同源性。实时定量 RT-PCR 分析表明，这些 Gh14-3-3 基因在纤维中的表达是发育调控的，在纤维发育的快速细胞伸长阶段达到峰值。此外，裂殖酵母中 Gh14-3-3a、Gh14-3-3e 和 Gh14-3-3L 的过表达促进了宿主细胞的非典型纵向生长。酵母双杂交分析表明，棉花 14-3-3 蛋白的相互作用是同工型选择性的。通过酵母双杂交筛选，从棉花纤维中鉴定出 6 种 14-3-3 蛋白（Gh14-3-3a、Gh14-3-3e、Gh14-3-3f、Gh14-3-3g、Gh14-3-3h 和 Gh14-3-3L）的 38 个新的相互作用伙伴，这些蛋白参与植物发育、代谢、信号转导和其他细胞过程。综合这些数据，提出 Gh14-3-3 蛋白可能参与调节纤维细胞伸长。因此，本研究结果为棉花纤维发育的 14-3-3 信号提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/b1029adbbe98/jexboterq155f10_ht.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/4ef9091d0012/jexboterq155f01_ht.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/01b870e767f3/jexboterq155f02_lw.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/ddcc171c13ba/jexboterq155f03_lw.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/4e86788a1c78/jexboterq155f04_lw.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/e9c555b27fee/jexboterq155f05_lw.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/0a43fc0d8dc1/jexboterq155f06_ht.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/849af7ab7614/jexboterq155f07_ht.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/0a01875702e9/jexboterq155f08_ht.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/6916759908aa/jexboterq155f09_ht.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/b1029adbbe98/jexboterq155f10_ht.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/4ef9091d0012/jexboterq155f01_ht.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/01b870e767f3/jexboterq155f02_lw.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/ddcc171c13ba/jexboterq155f03_lw.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/4e86788a1c78/jexboterq155f04_lw.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/e9c555b27fee/jexboterq155f05_lw.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/0a43fc0d8dc1/jexboterq155f06_ht.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/849af7ab7614/jexboterq155f07_ht.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/0a01875702e9/jexboterq155f08_ht.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/6916759908aa/jexboterq155f09_ht.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f712/2905198/b1029adbbe98/jexboterq155f10_ht.jpg

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