Key Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, Beijing, 100102, China.
Mol Biol Rep. 2022 Sep;49(9):8815-8825. doi: 10.1007/s11033-022-07731-4. Epub 2022 Jul 22.
As a ubiquitous acid-regulating protein family in eukaryotes, general regulatory factors (GRFs) are active in various life activities of plants. However, detailed investigations of the GRFs gene family in moso bamboo are scarce.
Genome-wide characteristics of the GRF gene family in moso bamboo were analyzed using the moso bamboo genome. GRF phylogeny, gene structure, conserved domains, cis-element promoters, and gene expression were systematically analyzed. A total of 20 GRF gene family members were identified in the moso bamboo genome. These genes were divided into ε and non-ε groups. qRT-PCR (real-time quantitative reverse transcription polymerase chain reaction) showed that PheGRF genes responded to auxin and gibberellin treatment. To further study PheGRF gene functions, a yeast two-hybrid experiment was performed and verified by a bimolecular fluorescence complementation experiment. The results showed that PheGRF4e could interact with PheIAA30 (auxin/indole-3-acetic acid, an Aux/IAA family gene), and both were found to act mainly on the root tip meristem and vascular bundle cells of developing shoots by in situ hybridization assay.
This study revealed that PheGRF genes were involved in hormone response during moso bamboo shoot development, and the possible regulatory functions of PheGRF genes were enriched by the fact that PheGRF4e initiated auxin signaling by binding to PheIAA30.
作为真核生物中普遍存在的酸调节蛋白家族，一般调节因子（GRF）在植物的各种生命活动中都很活跃。然而，毛竹 GRF 基因家族的详细研究还很少。
利用毛竹基因组分析了毛竹中 GRF 基因家族的全基因组特征。系统分析了 GRF 系统发育、基因结构、保守结构域、顺式元件启动子和基因表达。在毛竹基因组中鉴定出 20 个 GRF 基因家族成员。这些基因分为 ε 和非-ε 两组。qRT-PCR（实时定量逆转录聚合酶链反应）显示 PheGRF 基因对生长素和赤霉素处理有反应。为了进一步研究 PheGRF 基因的功能，进行了酵母双杂交实验，并通过双分子荧光互补实验进行了验证。结果表明，PheGRF4e 可以与 PheIAA30（生长素/吲哚-3-乙酸，Aux/IAA 家族基因）相互作用，通过原位杂交实验发现两者主要作用于竹笋发育顶端分生组织和维管束细胞。
本研究表明，PheGRF 基因参与了毛竹笋发育过程中的激素反应，通过 PheGRF4e 与 PheIAA30 结合启动生长素信号，丰富了 PheGRF 基因的可能调节功能。
