Korb J, Heine U
Arch Virol. 1978;56(3):211-25. doi: 10.1007/BF01317850.
Ribosomal RNA isolated from ribosomes present inside avian myeloblastosis virus (AMV) was characterized by electron microscopy using the formamide-urea spreading technique. The molecular weight and the secondary structures were compared with those of r-RNA and precursor r-NA isolated from host cells, the leukemic myeloblasts. The molecular weight of viral r-RNA (1.62 +/- 0.18 X 10(6) and 0.69 +/- 0.10 X 10(6)) and the molecular weight of cellular r-RNA (1.63 +/- 0.18 X 10(6) and 0.67 +/- 0.09 X 10(6)), the latter obtained from avian myeloblasts, were found to be identical and comparable with the molecular weight of chicken liver r-RNA. Likewise, the secondary structures of viral r-RNA were identical to those of cellular r-RNA. The postulated possible precursor character of viral r-RNA was excluded, since the molecules of viral r-RNA do not show any similarity to those of precursor r-RNA. Previously observed differences in behavior of viral and cellular (myeloblastic) r-RNA in sedimentation and electrophoretic mobility are discussed.
采用甲酰胺-尿素铺展技术，通过电子显微镜对从禽成髓细胞瘤病毒（AMV）内部存在的核糖体中分离出的核糖体RNA进行了表征。将其分子量和二级结构与从宿主细胞即白血病成髓细胞中分离出的r-RNA和前体r-NA的分子量和二级结构进行了比较。病毒r-RNA的分子量（1.62±0.18×10⁶和0.69±0.10×10⁶）以及细胞r-RNA的分子量（1.63±0.18×10⁶和0.67±0.09×10⁶，后者从禽成髓细胞中获得），被发现与鸡肝r-RNA的分子量相同且具有可比性。同样，病毒r-RNA的二级结构与细胞r-RNA的二级结构相同。病毒r-RNA假定的可能的前体特征被排除，因为病毒r-RNA的分子与前体r-RNA的分子没有任何相似性。讨论了先前观察到的病毒和细胞（成髓细胞性）r-RNA在沉降和电泳迁移率方面行为的差异。
