通过分子杂交测定禽肿瘤病毒前病毒DNA的同质性和复杂性。
Homogeneity and complexity of avian oncornavirus proviral DNA determined by molecular hybridization.
作者信息
Evans R M, Shoyab M, Drohan W N, Baluda M A
出版信息
J Virol. 1977 Mar;21(3):942-9. doi: 10.1128/JVI.21.3.942-949.1977.
Abstract
The homogeneity of DNA complementary to the 35S RNA subunit of avian myeloblastosis virus (AMV) has been demonstrated by single or multistep hybridization. For multistep hybridizations, 35S AMV RNA was preselected for its ability to hybridize either to unfractionated leukemic DNA or to leukemic DNA enriched for unique or for reiterated sequences. These experiments indicate that the viral genome is complementary to DNA sequences with a low reiteration frequency. Competition experiments confirm the absence of fast-hybridizing sequences in viral DNA. Computer analyses of the data reveal that there are two to four copies of viral DNA in infected cells.
摘要
通过单步或多步杂交已证明与禽成髓细胞瘤病毒（AMV）35S RNA亚基互补的DNA具有同质性。对于多步杂交，根据35S AMV RNA与未分级的白血病DNA或富含单一或重复序列的白血病DNA杂交的能力对其进行预筛选。这些实验表明，病毒基因组与具有低重复频率的DNA序列互补。竞争实验证实病毒DNA中不存在快速杂交序列。对数据的计算机分析表明，感染细胞中有两到四个病毒DNA拷贝。
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